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GO:0075004adhesion of symbiont spore to hostPMID:25605761IDA: Inferred from Direct Assay P
This annotation made on page: NOSB1:SWP7
By: Min.lorna (group Team Ood) on 2015-02-10 17:31:15 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeMin.lorna,
Team Ood
2015-03-02 13:35:09 CST

Evidence code should be IMP - recombinant protein was found to bind to BmE cells. Fig. 8A: B3 - BmE cells were first incubated with recombinant SWP7 and then anti-SWP7 - BmE cell surfaces fluoresced aftewards. BmE cells were also incubated with DAPI so BmE nuclei could be visualized - SWP7 overlapped with nuclei, indicating SWP7 binds to BmE cells

0
ChallengeKsenagbe,
Team Mic Drop
2015-02-17 15:24:44 CST

Incomplete note. Need to add that the results shows the identification and characterization of two novel spore wall proteins, SWP7 and SWP9, in the microsporidian species Nosema bombycis. Further more, the far-western blotting, unreduced SDS-PAGE and yeast two hybrid data demonstrated that SWP7 interacted with SWP9. The chitin binding assay showed that within the total spore protein, SWP9 and SWP7 can bind to the deproteinated chitin spore coats (DCSCs) of N. bombycis.

0
Private
Assessment
Suzialeksander2015-03-03 20:21:54 CSTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Private
Assessment
Suzialeksander2015-03-03 18:54:41 CSTYou need to be an instructor to view these notes.Flagged
Public
Assessment
DanielRenfro2015-03-02 13:35:09 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
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Assessment
Suzialeksander2015-02-26 12:58:48 CST

Ksenagbe, you summarised the paper well. However, you need to refer to specific information that supports the annotation, not just what the source said and did in general. I can read the abstract to know what the paper said; your job is to prove the paper supports this GO term with a conclusive figure or table.

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Private
Assessment
Suzialeksander2015-02-26 12:52:12 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original