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STREE:O85255

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Species (Taxon ID) Streptococcus pneumoniae. (1313)
Gene Name(s) dfr (ECO:0000313 with EMBL:AAC33861.1) (synonyms: dhfr (ECO:0000313 with EMBL:AAG22523.1))
Protein Name(s) Dihydrofolate reductase (ECO:0000256 with PIRNR:PIRNR000194)
External Links
UniProt O85255
EMBL AF055720
AF288414
AF288418
ProteinModelPortal O85255
UniPathway UPA00077
GO GO:0004146
GO:0050661
GO:0006545
GO:0009165
GO:0006730
GO:0046654
Gene3D 3.40.430.10
InterPro IPR012259
IPR024072
IPR017925
IPR001796
Pfam PF00186
PIRSF PIRSF000194
PRINTS PR00070
SUPFAM SSF53597
PROSITE PS00075
PS51330

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004146

dihydrofolate reductase activity

PMID:19950924[1]

ECO:0000315

F

Figure 8 shows how the mutation of Val100 to Leu makes the leucine side chain clash with side chains of Ile8 and Phe34, shifting their positions and stacking interactions with the trimethoprim molecule. "Weaker binding of trimethoprim to Leu100 DHFR would lead to trimethoprim resistance". The KM values of NADPH and H2F for the wild type spDHFR and the mutant Sp9 were measured (Table 2B). The KM values for the wild type spDHFR were at least order of magnitude higher than those for the Sp9 mutant that showed full enzyme activity even at a limiting low concentration of 0.63 μM H2F consistent with a much lower KM value for this substrate.

complete
CACAO 10955

enables

GO:0004146

dihydrofolate reductase activity

PMID:19950924[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0004146

dihydrofolate reductase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR001796
InterPro:IPR012259
InterPro:IPR017925

F

Seeded From UniProt

complete

involved_in

GO:0006545

glycine biosynthetic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR012259

P

Seeded From UniProt

complete

involved_in

GO:0046654

tetrahydrofolate biosynthetic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR001796
InterPro:IPR012259

P

Seeded From UniProt

complete

enables

GO:0050661

NADP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR012259

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR001796
InterPro:IPR012259
InterPro:IPR017925

P

Seeded From UniProt

complete

enables

GO:0004146

dihydrofolate reductase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:1.5.1.3

F

Seeded From UniProt

complete

enables

GO:0016491

oxidoreductase activity

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0560

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0560

P

Seeded From UniProt

complete

involved_in

GO:0006730

one-carbon metabolic process

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0554

P

Seeded From UniProt

complete

involved_in

GO:0046654

tetrahydrofolate biosynthetic process

GO_REF:0000041

ECO:0000322

imported manually asserted information used in automatic assertion

UniPathway:UPA00077

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Lee, J et al. (2010) Kinetic and structural characterization of dihydrofolate reductase from Streptococcus pneumoniae. Biochemistry 49 195-206 PubMed GONUTS page