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SCHPO:MTF1
Contents
| Species (Taxon ID) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812) | |
| Gene Name(s) | mtf1 | |
| Protein Name(s) | Mitochondrial transcription factor 1 | |
| External Links | ||
| UniProt | Q9US51 | |
| EMBL | CU329670 | |
| RefSeq | NP_593495.1 | |
| ProteinModelPortal | Q9US51 | |
| SMR | Q9US51 | |
| BioGrid | 279699 | |
| STRING | 4896.SPAC1002.08c.1 | |
| MaxQB | Q9US51 | |
| PaxDb | Q9US51 | |
| PRIDE | Q9US51 | |
| EnsemblFungi | SPAC1002.08c.1 | |
| GeneID | 2543271 | |
| KEGG | spo:SPAC1002.08c | |
| EuPathDB | FungiDB:SPAC1002.08c | |
| PomBase | SPAC1002.08c | |
| InParanoid | Q9US51 | |
| KO | K15267 | |
| OMA | CKLSVIA | |
| PhylomeDB | Q9US51 | |
| PRO | PR:Q9US51 | |
| Proteomes | UP000002485 | |
| GO | GO:0034245 GO:0005759 GO:0005739 GO:0000790 GO:0005634 GO:0003677 GO:0003723 GO:0016433 GO:1903109 GO:0045944 GO:0006391 | |
| Gene3D | 1.10.8.100 | |
| InterPro | IPR001737 IPR023165 IPR029063 | |
| PANTHER | PTHR11727 | |
| Pfam | PF00398 | |
| SUPFAM | SSF53335 | |
| PROSITE | PS51689 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0005739 |
mitochondrion |
ECO:0000314 |
C |
Source: GeneDB_Spombe |
Missing: reference | |||||
| GO:0005634 |
nucleus |
ECO:0000269 |
C |
Figure 1B shows the location of the intense fluorescence by comparison with DAPI and DiOC6 suggesting nuclear localization.Figure 1D shows that the Mtf1-GFP signal superimposes on the nuclear signal from DAPI(nuclear stain) confirming that Mtf1-GFP localizes to the nucleus. |
complete | |||||
| GO:0045449 |
regulation of transcription |
ECO:0000315 |
P |
Figure 2A shows the RT-PCR of srk1 transcripts abundance in cells transformed with vector only and cells with pREP3-Mtf1 overexpressing Mtf1 in the absence of Thiamine.It also shows that transcription of srk1 is reduced in Mtf1 deletion mutant cells. |
complete | |||||
| GO:0010628 |
positive regulation of gene expression |
ECO:0000269 |
P |
Figure 3D shows that the srk1 protein levels are induced when Mtf1 expression is induced in the absence of thiamine. |
complete | |||||
| GO:0003700 |
sequence-specific DNA binding transcription factor activity |
ECO:0000314 |
F |
Figure 5G shows the EMSA with the upstream of the srk1 DNA probe and a sequence taken from the coding sequence of the srk1 gene (CDS) as a control with and without Mtf1 and confirms that Mtf1 specifically binds to srk1 promoter sequence. |
complete | |||||
| GO:0001018 |
mitochondrial RNA polymerase regulatory region DNA binding |
ECO:0000314 |
F |
Figure 5F shows the EMSA with a mitochondrial promoter Pmin with and without Mtf1 and thus confirms regulatory region DNA binding of transcription factor mtf1. |
complete | |||||
|
involved_in |
GO:1903109 |
positive regulation of mitochondrial transcription |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1903109 |
positive regulation of mitochondrial transcription |
ECO:0000269 |
experimental evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
has_regulation_target:(PomBase:SPCC1322.08) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0034245 |
mitochondrial DNA-directed RNA polymerase complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016433 |
rRNA (adenine) methyltransferase activity |
ECO:0000255 |
match to sequence model evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0005739 |
mitochondrion |
ECO:0000314 |
C |
complete | ||||||
|
part_of |
GO:0005759 |
mitochondrial matrix |
ECO:0000266 |
sequence orthology evidence used in manual assertion |
SGD:S000004841 |
C |
Seeded From UniProt |
complete | ||
| GO:0005739 |
mitochondrion |
ECO:0000314 |
C |
complete | ||||||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0005739 |
mitochondrion |
ECO:0000315 |
SGD:S000004841 |
C |
complete | |||||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0005759 |
mitochondrial matrix |
ECO:0000250 |
SGD:S000004841 |
C |
complete | |||||
|
part_of |
GO:0000790 |
nuclear chromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0034245 |
mitochondrial DNA-directed RNA polymerase complex |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001712033 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006391 |
transcription initiation from mitochondrial promoter |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001712033 |
P |
Seeded From UniProt |
complete | ||
| GO:0006390 |
transcription from mitochondrial promoter |
ECO:0000315 |
SGD:S000004841 |
P |
complete | |||||
|
part_of |
GO:0005759 |
mitochondrial matrix |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000197847 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0031167 |
rRNA methylation |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0016433 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739 |
mitochondrion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008168 |
methyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032259 |
methylation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Sun, W et al. (2011) A novel function of the mitochondrial transcription factor Mtf1 in fission yeast; Mtf1 regulates the nuclear transcription of srk1. Nucleic Acids Res. 39 2690-700 PubMed GONUTS page
- ↑ 2.0 2.1 Jiang, H et al. (2011) Identification and characterization of the mitochondrial RNA polymerase and transcription factor in the fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 39 5119-30 PubMed GONUTS page
- ↑ 3.0 3.1 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
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