It is now the 3rd CHALLENGE Period for CACAO Fall 2017! It will end on Sunday October 22, 2017 at 11:59 pm CDT
This is your chance to challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

Have any questions? Please email us at ecoliwiki@gmail.com

SCHPO:MTF1

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Schizosaccharomyces pombe (strain ATCC 38366 / 972) (Fission yeast). (taxon:284812)
Gene Name(s) mtf1
Protein Name(s)
  • Mitochondrial transcription factor 1
External Links
UniProt Identifier MTF1_SCHPO
UniProt Accessions Q9US51,
EMBL CU329670,
RefSeq NP_593495.1,
Pfam PF00398,

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005739

mitochondrion

IDA: Inferred from Direct Assay

C

Source: GeneDB_Spombe

GO:0003677

DNA binding

IEA: Inferred from Electronic Annotation

F

Source: UniProtKB-KW

GO:0000179

rRNA (adenine-N6,N6-)-dimethyltransferase a...

IEA: Inferred from Electronic Annotation

F

Source: InterPro

GO:0045449

regulation of transcription

IEA: Inferred from Electronic Annotation

P

Source: UniProtKB-KW

GO:0006350

transcription

IEA: Inferred from Electronic Annotation

P

Source: UniProtKB-KW

GO:0005634

nucleus

PMID:21138961[1]

EXP: Inferred from Experiment

C

Figure 1B shows the location of the intense fluorescence by comparison with DAPI and DiOC6 suggesting nuclear localization.Figure 1D shows that the Mtf1-GFP signal superimposes on the nuclear signal from DAPI(nuclear stain) confirming that Mtf1-GFP localizes to the nucleus.

complete

GO:0045449

regulation of transcription

PMID:21138961[1]

IMP: Inferred from Mutant Phenotype

P

Figure 2A shows the RT-PCR of srk1 transcripts abundance in cells transformed with vector only and cells with pREP3-Mtf1 overexpressing Mtf1 in the absence of Thiamine.It also shows that transcription of srk1 is reduced in Mtf1 deletion mutant cells.

complete

GO:0010628

positive regulation of gene expression

PMID:21138961[1]

EXP: Inferred from Experiment

P

Figure 3D shows that the srk1 protein levels are induced when Mtf1 expression is induced in the absence of thiamine.

complete

GO:0003700

sequence-specific DNA binding transcription factor activity

PMID:21138961[1]

IDA: Inferred from Direct Assay

F

Figure 5G shows the EMSA with the upstream of the srk1 DNA probe and a sequence taken from the coding sequence of the srk1 gene (CDS) as a control with and without Mtf1 and confirms that Mtf1 specifically binds to srk1 promoter sequence.

complete

GO:0001018

mitochondrial RNA polymerase regulatory region DNA binding

PMID:21138961[1]

IDA: Inferred from Direct Assay

F

Figure 5F shows the EMSA with a mitochondrial promoter Pmin with and without Mtf1 and thus confirms regulatory region DNA binding of transcription factor mtf1.

complete

GO:0000154

rRNA modification

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001737

P

GO:0000179

rRNA (adenine-N6,N6-)-dimethyltransferase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001737

F

GO:0000980

RNA polymerase II enhancer sequence-specific DNA binding

PMID:21138961[1]

IDA: Inferred from Direct Assay

F

GO:0003677

DNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

GO:0005634

nucleus

PMID:21138961[1]

IDA: Inferred from Direct Assay

C

GO:0005739

mitochondrion

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0496

C

GO:0005739

mitochondrion

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0173

C

GO:0005739

mitochondrion

PMID:16823372[2]

IDA: Inferred from Direct Assay

C

GO:0005739

mitochondrion

PMID:21138961[1]

IDA: Inferred from Direct Assay

C

GO:0005739

mitochondrion

PMID:21138961[1]

IMP: Inferred from Mutant Phenotype

SGD:S000004841

C

GO:0005759

mitochondrial matrix

GO_REF:0000001

ISS: Inferred from Sequence or Structural Similarity

SGD:S000004841

C

GO:0006351

transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

GO:0006355

regulation of transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

GO:0006357

regulation of transcription from RNA polymerase II promoter

PMID:21138961[1]

IDA: Inferred from Direct Assay

P

GO:0006390

transcription from mitochondrial promoter

PMID:21138961[1]

IMP: Inferred from Mutant Phenotype

SGD:S000004841

P

GO:0008649

rRNA methyltransferase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001737

F

GO:0008757

S-adenosylmethionine-dependent methyltransferase activity

GO_REF:0000001

ISS: Inferred from Sequence or Structural Similarity

SGD:S000004841

F

GO:0031167

rRNA methylation

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001737

P


Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Sun, W et al. (2011) A novel function of the mitochondrial transcription factor Mtf1 in fission yeast; Mtf1 regulates the nuclear transcription of srk1. Nucleic Acids Res. 39 2690-700 PubMed GONUTS page
  2. Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page