PSEAE:G3XCY4

Species (Taxon ID)	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228). (208964)
Gene Name(s)	amrZ (ECO:0000313 with EMBL:AAG06773.1)
Protein Name(s)	Alginate and motility regulator Z (ECO:0000313 with EMBL:AAG06773.1)
External Links
UniProt	G3XCY4
EMBL	AE004091
PIR	D83221
RefSeq	NP_252075.1
PDB	3QOQ
PDBsum	3QOQ
ProteinModelPortal	G3XCY4
SMR	G3XCY4
DNASU	878714
EnsemblBacteria	AAG06773
GeneID	878714
KEGG	pae:PA3385
PseudoCAP	PA3385
OMA	EQSMLQE
OrthoDB	EOG68H89S
Proteomes	UP000002438
GO	GO:0003677 GO:0044212 GO:2000147 GO:1900233 GO:0006355
Gene3D	1.10.1220.10
InterPro	IPR005569 IPR013321 IPR010985
Pfam	PF03869
SUPFAM	SSF47598

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0009405	pathogenesis	PMID:20709902^[1]	ECO:0000315			P	Figure 6 demonstrates that AmrZ mutants have reduced virulence in mice.	complete CACAO 10082
involved_in	GO:0009405	pathogenesis	PMID:20709902^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0045892	negative regulation of transcription, DNA-templated	PMID:23354748^[2]	ECO:0005581	enzyme-linked immunoabsorbent assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0045892	negative regulation of transcription, DNA-templated	PMID:23354748^[2]	ECO:0001808	reverse transcription polymerase chain reaction evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0045892	negative regulation of transcription, DNA-templated	PMID:23354748^[2]	ECO:0000279	western blot evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:22511872^[3]	ECO:0005670	x-ray crystallography evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:22511872^[3]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:22511872^[3]	ECO:0005632	fluorescence anisotropy evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:24603766^[4]	ECO:0006009	chromatin immunoprecipitation-seq evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:22511872^[3]	ECO:0005670	x-ray crystallography evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:22511872^[3]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:22511872^[3]	ECO:0005632	fluorescence anisotropy evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:15968052^[5]	ECO:0005626	copper-phenanthroline footprinting evidence used in manual assertion	RefSeq:NC_002516.2	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:24603766^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:23354748^[2]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:15968052^[5]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:24603766^[4]	ECO:0006009	chromatin immunoprecipitation-seq evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:15968052^[5]	ECO:0005626	copper-phenanthroline footprinting evidence used in manual assertion	RefSeq:NC_002516.2	F	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:24603766^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:23354748^[2]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001217	DNA-binding transcription repressor activity	PMID:15968052^[5]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:24603766^[4]	ECO:0006009	chromatin immunoprecipitation-seq evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:24603766^[4]	ECO:0006009	chromatin immunoprecipitation-seq evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:15968052^[5]	ECO:0005626	copper-phenanthroline footprinting evidence used in manual assertion	RefSeq:NC_002516.2	F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:24603766^[4]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:23354748^[2]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:15968052^[5]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:2000147	positive regulation of cell motility	PMID:16352829^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:1902201	negative regulation of bacterial-type flagellum-dependent cell motility	PMID:16952938^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:1900233	positive regulation of single-species biofilm formation on inanimate substrate	PMID:10476040^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:1900232	negative regulation of single-species biofilm formation on inanimate substrate	PMID:24603766^[4]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0044780	bacterial-type flagellum assembly	PMID:16952938^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0044212	transcription regulatory region DNA binding	PMID:16952938^[7]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0043107	type IV pilus-dependent motility	PMID:16352829^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0032885	regulation of polysaccharide biosynthetic process	PMID:23354748^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0032885	regulation of polysaccharide biosynthetic process	PMID:10476040^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009405	pathogenesis	PMID:20709902^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	PMID:16352829^[6]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001150	bacterial-type RNA polymerase enhancer sequence-specific DNA binding	PMID:8899712^[9]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000986	bacterial-type proximal promoter sequence-specific DNA binding	PMID:24603766^[4]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0001216	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005569	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR010985 InterPro:IPR013321	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 Waligora, EA et al. (2010) AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes. J. Bacteriol. 192 5390-401 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 Jones, CJ et al. (2013) AmrZ modulates Pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon. J. Bacteriol. 195 1637-44 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 Pryor, EE Jr et al. (2012) The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes. PLoS Pathog. 8 e1002648 PubMed GONUTS page
↑ ^4.0 ^4.1 ^4.2 ^4.3 ^4.4 ^4.5 ^4.6 ^4.7 ^4.8 Jones, CJ et al. (2014) ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa. PLoS Pathog. 10 e1003984 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 Ramsey, DM et al. (2005) Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription. J. Bacteriol. 187 4430-43 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 Baynham, PJ et al. (2006) The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV pili. J. Bacteriol. 188 132-40 PubMed GONUTS page
↑ ^7.0 ^7.1 ^7.2 Tart, AH et al. (2006) The AlgT-dependent transcriptional regulator AmrZ (AlgZ) inhibits flagellum biosynthesis in mucoid, nonmotile Pseudomonas aeruginosa cystic fibrosis isolates. J. Bacteriol. 188 6483-9 PubMed GONUTS page
↑ ^8.0 ^8.1 Baynham, PJ et al. (1999) Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation. Mol. Microbiol. 33 1069-80 PubMed GONUTS page
↑ Baynham, PJ & Wozniak, DJ (1996) Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription. Mol. Microbiol. 22 97-108 PubMed GONUTS page

[PMID:20709902-1] 1.0 ^1.1 ^1.2 Waligora, EA et al. (2010) AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes. J. Bacteriol. 192 5390-401 PubMed GONUTS page

[PMID:23354748-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 Jones, CJ et al. (2013) AmrZ modulates Pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon. J. Bacteriol. 195 1637-44 PubMed GONUTS page

[PMID:22511872-3] 3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 Pryor, EE Jr et al. (2012) The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes. PLoS Pathog. 8 e1002648 PubMed GONUTS page

[PMID:24603766-4] 4.0 ^4.1 ^4.2 ^4.3 ^4.4 ^4.5 ^4.6 ^4.7 ^4.8 Jones, CJ et al. (2014) ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa. PLoS Pathog. 10 e1003984 PubMed GONUTS page

[PMID:15968052-5] 5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 Ramsey, DM et al. (2005) Binding of Pseudomonas aeruginosa AlgZ to sites upstream of the algZ promoter leads to repression of transcription. J. Bacteriol. 187 4430-43 PubMed GONUTS page

[PMID:16352829-6] 6.0 ^6.1 ^6.2 Baynham, PJ et al. (2006) The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV pili. J. Bacteriol. 188 132-40 PubMed GONUTS page

[PMID:16952938-7] 7.0 ^7.1 ^7.2 Tart, AH et al. (2006) The AlgT-dependent transcriptional regulator AmrZ (AlgZ) inhibits flagellum biosynthesis in mucoid, nonmotile Pseudomonas aeruginosa cystic fibrosis isolates. J. Bacteriol. 188 6483-9 PubMed GONUTS page

[PMID:10476040-8] 8.0 ^8.1 Baynham, PJ et al. (1999) Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation. Mol. Microbiol. 33 1069-80 PubMed GONUTS page

[PMID:8899712-9] Baynham, PJ & Wozniak, DJ (1996) Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription. Mol. Microbiol. 22 97-108 PubMed GONUTS page

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PSEAE:G3XCY4

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