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PMID:9786189
| Citation |
Okamoto, S and Ochi, K (1998) An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor. Mol. Microbiol. 30:107-19 |
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| Abstract |
The Streptomyces coelicolor obg gene, which encodes a putative GTP-binding protein of the Obg/Gtp1 family, was characterized. The obg gene was essential for viability. Introduction of multiple copies of obg into wild-type S. coelicolor suppressed aerial mycelium formation. A single amino acid substitution at any of six positions was introduced into the GTP binding site of Obg, and the mutated proteins were expressed in wild-type cells. Obg(P168-->V) exerted a more accentuated suppressive effect on aerial mycelium formation than did the wild-type Obg protein. In contrast, Obg(G171-->A) accelerated the development of aerial mycelium. These results show that Obg protein functions as a pivotal regulator for the onset of cell differentiation through its ability to bind GTP. Western analysis revealed that expression of obg is regulated in a growth phase-dependent manner, indicating a sharp decrease just after onset of aerial mycelium development or at the end of vegetative growth. Obg was a membrane-bound protein as determined by immunoelectron microscopy. |
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| Keywords |
Bacterial Proteins/analysis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Western; GTP-Binding Proteins/analysis; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Guanosine Triphosphate/metabolism; Microscopy, Immunoelectron; Mutagenesis, Site-Directed; Physical Chromosome Mapping; Recombination, Genetic; Streptomyces/cytology; Streptomyces/genetics; Streptomyces/growth & development; Streptomyces/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006184: GTP catabolic process |
ECO:0000315: |
P |
Figure 8: It is proposed that a decrease in the GTP-bound form of Obg is a crucial event for triggering development in Streptomyces coelicolor. |
complete | ||||
See also
References
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