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PMID:9751729
| Citation |
Avdonin, V, Kasuya, J, Ciorba, MA, Kaplan, B, Hoshi, T and Iverson, L (1998) Apoptotic proteins Reaper and Grim induce stable inactivation in voltage-gated K+ channels. Proc. Natl. Acad. Sci. U.S.A. 95:11703-8 |
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| Abstract |
Drosophila genes reaper, grim, and head-involution-defective (hid) induce apoptosis in several cellular contexts. N-terminal sequences of these proteins are highly conserved and are similar to N-terminal inactivation domains of voltage-gated potassium (K+) channels. Synthetic Reaper and Grim N terminus peptides induced fast inactivation of Shaker-type K+ channels when applied to the cytoplasmic side of the channel that was qualitatively similar to the inactivation produced by other K+ channel inactivation particles. Mutations that reduce the apoptotic activity of Reaper also reduced the synthetic peptide's ability to induce channel inactivation, indicating that K+ channel inactivation correlated with apoptotic activity. Coexpression of Reaper RNA or direct injection of full length Reaper protein caused near irreversible block of the K+ channels. These results suggest that Reaper and Grim may participate in initiating apoptosis by stably blocking K+ channels. |
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| Keywords |
Amino Acid Sequence; Animals; Apoptosis/genetics; Apoptosis/physiology; Base Sequence; DNA Primers/genetics; Drosophila/cytology; Drosophila/genetics; Drosophila/metabolism; Drosophila Proteins; Female; Genes, Insect; Insect Proteins/genetics; Insect Proteins/metabolism; Molecular Sequence Data; Mutation; Neuropeptides/genetics; Neuropeptides/metabolism; Oocytes/metabolism; Peptide Fragments/genetics; Peptide Fragments/metabolism; Peptide Fragments/pharmacology; Peptides/genetics; Peptides/metabolism; Potassium Channel Blockers; Potassium Channels/genetics; Potassium Channels/metabolism; Recombinant Proteins/antagonists & inhibitors; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Shaker Superfamily of Potassium Channels; Xenopus |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0008627: intrinsic apoptotic signaling pathway in response to osmotic stress |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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