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PMID:9731187
| Citation |
Motoyama, S, Kitamura, M, Saito, S, Minamiya, Y, Suzuki, H, Saito, R, Terada, K, Ogawa, J and Inaba, H (1998) Bcl-2 is located predominantly in the inner membrane and crista of mitochondria in rat liver. Biochem. Biophys. Res. Commun. 249:628-36 |
|---|---|
| Abstract |
Bcl-2 is now recognized as a potent inhibitor of apoptotic cell death. It has been reported that Bcl-2 is located in the mitochondria, endoplasmic reticulum, and nuclear membrane in some cell lines, and it is not expressed in normal human and rat liver. An earlier study showed that Bcl-2 is an inner mitochondrial membrane protein. On the contrary, the following investigations using immunoelectron microscopy demonstrated that Bcl-2 resides predominantly in the mitochondrial outer membrane. In this study, using a cryo-sectioning immunogold labeling technique and immunoblotting, we carefully determined the subcellular localization of Bcl-2. Here we report that Bcl-2 is expressed in normal rat liver, and it is located predominantly in the inner membrane and crista rather than in the outer membrane of mitochondria. |
| Links |
PubMed Online version:10.1006/bbrc.1998.9205 |
| Keywords |
Animals; Cell Fractionation; HL-60 Cells; Humans; Intracellular Membranes/metabolism; Intracellular Membranes/ultrastructure; Male; Microscopy, Immunoelectron; Mitochondria, Liver/metabolism; Mitochondria, Liver/ultrastructure; Proto-Oncogene Proteins c-bcl-2/metabolism; Rats; Rats, Sprague-Dawley; Submitochondrial Particles/metabolism; Submitochondrial Particles/ultrastructure |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0030061: mitochondrial crista |
ECO:0000314: |
C |
Figure 6 clearly illustrates that Bcl-2 is predominantly localized in the crista of the Mitochondria. Uniprot Nomenclature: Apoptosis regulator Bcl-2 (BCL2_RAT) Uniprot ID: P49950 Organism: Rattus norvegicus |
complete | ||||
| GO:0005743: mitochondrial inner membrane |
ECO:0000314: |
C |
Figure 6 clearly illustrates that Bcl-2 is predominantly localized in the inner mitochondrial membrane. Uniprot Nomenclature: Apoptosis regulator Bcl-2 (BCL2_RAT) Uniprot ID: P49950 Organism: Rattus norvegicus |
complete | ||||
Notes
See also
References
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