PMID:9701813

Citation	Bsat, N, Herbig, A, Casillas-Martinez, L, Setlow, P and Helmann, JD (1998) Bacillus subtilis contains multiple Fur homologues: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors. Mol. Microbiol. 29:189-98
Abstract	Fur (ferric uptake regulator) proteins control iron uptake in many Gram-negative bacteria. Although Fur homologues have been identified in Gram-positive bacteria, their roles in gene regulation are unknown. Genome sequencing has revealed three fur homologues in Bacillus subtilis: yqkL, yqfV and ygaG. We demonstrate that yqkL encodes an iron uptake repressor: both siderophore biosynthesis and transcription of ferri-siderophore uptake genes is constitutive in the yqkL mutant. Thus, yqkL encodes a repressor that is functionally as well as structurally related to Fur. B. subtilis peroxide stress genes are induced by either H2O2 or by metal ion limitation. Previous genetic studies defined a regulatory locus, perR, postulated to encode the peroxide regulon repressor. We demonstrate that a ygaG mutant has the perR phenotype: It is highly resistant to peroxides and overexpresses catalase, alkyl hydroperoxide reductase and the DNA binding protein MrgA. Nine spontaneous perR mutations, isolated by virtue of their ability to derepress mrgA transcription in the presence of managanous ion, all contain sequence changes in the ygaG locus and can be complemented by the cloned ygaG gene. Thus, ygaG encodes the peroxide regulon repressor and is allelic with perR.
Links	PubMed
Keywords	Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Gene Expression Regulation, Bacterial; Hydroxybenzoic Acids/metabolism; Iron/metabolism; Molecular Sequence Data; Oxidative Stress; Peroxides/metabolism; Regulon; Repressor Proteins/genetics; Repressor Proteins/metabolism; Siderophores/metabolism; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:FUR	involved_in	GO:0045892: negative regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
BACSU:FUR		GO:0045892: negative regulation of transcription, DNA-dependent	ECO:0000315:		P		As the iron-dependent repression of both catechol siderophore synthesis (Fig. 2) and hydroxamate siderophore uptake genes (Fig. 3A) is dependent on yqkL, we conclude that yqkL encodes a ferric uptake repressor protein, Fur. B. subtilis Fur recognizes operator sites similar to the consensus established for E. coli Fur.	complete CACAO 2741

References

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