PMID:9689123

Citation	Yuan, Y, Crane, DD, Simpson, RM, Zhu, YQ, Hickey, MJ, Sherman, DR and Barry, CE 3rd (1998) The 16-kDa alpha-crystallin (Acr) protein of Mycobacterium tuberculosis is required for growth in macrophages. Proc. Natl. Acad. Sci. U.S.A. 95:9578-83
Abstract	Although the 16-kDa alpha-crystallin homologue of Mycobacterium tuberculosis (MTB) is the dominant protein produced by stationary phase cultures in vitro, it is undetectable in logarithmically growing cultures. By growing bacilli at defined oxygen concentrations, acr transcription was shown to be strongly induced by mildly hypoxic conditions. Acr expression also was found to be induced during the course of in vitro infection of macrophages. The acr gene was replaced with a hygromycin resistance cassette by allelic exchange in MTB H37Rv. The resulting Deltaacr::hpt strain was shown to be equivalent to wild-type H37Rv in in vitro growth rate and infectivity but was significantly impaired for growth in both mouse bone marrow derived macrophages and THP-1 cells. In addition to its proposed role in maintenance of long-term viability during latent, asymptomatic infections, these results establish a role for the Acr protein in replication during initial MTB infection.
Links	PubMed PMC21381
Keywords	Animals; Crystallins/genetics; Crystallins/metabolism; Gene Expression Regulation, Bacterial; Macrophages/microbiology; Mice; Mycobacterium tuberculosis/genetics; Mycobacterium tuberculosis/growth & development; Mycobacterium tuberculosis/metabolism; Oxygen
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MYCTU:ACR	involved_in	GO:0044121: growth of symbiont in host organelle	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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