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PMID:9631512
| Citation |
Yang, Y and Lowe, ME (1998) Human pancreatic triglyceride lipase expressed in yeast cells: purification and characterization. Protein Expr. Purif. 13:36-40 |
|---|---|
| Abstract |
A cDNA clone encoding human pancreatic triglyceride lipase was cloned into a yeast expression vector so that the yeast PHO1 signal peptide replaced the native signal peptide. Pichia pastoris cells were transfected with the vector, and clones expressing human pancreatic triglyceride lipase were isolated. Recombinant human pancreatic lipase was expressed in broth cultures and was purified from the medium by DEAE blue Sepharose and hydroxyapatite chromatography. The highly purified lipase had specific activities for various triglyceride substrates identical to those of tissue-purified human pancreatic triglyceride lipase; it was inhibited by bile salts, required colipase for activity, and demonstrated interfacial activation. This expression system is suitable for the rapid, efficient production of human pancreatic triglyceride lipase in amounts adequate for biophysical studies. |
| Links |
PubMed Online version:10.1006/prep.1998.0874 |
| Keywords |
Amino Acid Sequence; Animals; Baculoviridae/genetics; Cell Line; Cloning, Molecular; Humans; Lipase/genetics; Lipase/isolation & purification; Lipase/metabolism; Pancreas/enzymology; Pichia/genetics; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spodoptera |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0061365: triglyceride lipase activity |
ECO:0000314: |
F |
Figure 1 |
complete | ||||
| GO:0004806: triglyceride lipase activity |
ECO:0000314: |
F |
Figure 1 |
complete | ||||
|
involved_in |
GO:0061365: positive regulation of triglyceride lipase activity |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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