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PMID:9504816
| Citation |
Scheler, C, Müller, EC, Stahl, J, Müller-Werdan, U, Salnikow, J and Jungblut, P (1997) Identification and characterization of heat shock protein 27 protein species in human myocardial two-dimensional electrophoresis patterns. Electrophoresis 18:2823-31 |
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| Abstract |
Immunostaining of heat shock protein 27 (Hsp27) protein species on two-dimensional electrophoresis (2-DE) gels with enhanced sensitivity yields 59 spots reacting with anti-Hsp27 antibodies. Recombinant Hsp27 exists in 2-DE as two major protein species which comigrate in the human myocardial pattern with Hsp27 spots C754 and D899 as defined in the heart high-performance 2-DE database (http://www.mdc-berlin.de/emu/heart/). Preparative electrophoresis of human myocardial proteins and analysis of the enriched mass range 20-30 kDa by 2-DE revealed eight protein spots (C438, C582, C658, C697, C754, C595, C750) from the human myocardial database and a new spot not previously detected on silver-stained gels. These spots were identified as Hsp27 protein species by enzymatic in-gel-digestion and analysis by matrix assisted laser desorption-ionization (MALDI) peptide mass fingerprinting and, in part, MALDI-post source decay sequencing of single fragments. Possible post-translational modifications were investigated: immunostaining tests with anti-phospho-serine/-threonine/-tyrosine antibodies, although positive for other myocardial proteins, were negative for presumed Hsp27 protein species; likewise, periodate-glycostaining assays and biotinylation screening did not detect modifications in the investigated Hsp27 protein species. |
| Links |
PubMed Online version:10.1002/elps.1150181518 |
| Keywords |
Amino Acid Sequence; Electrophoresis, Gel, Two-Dimensional; Heat-Shock Proteins/analysis; Heat-Shock Proteins/genetics; Humans; Immunoblotting; Indicators and Reagents; Molecular Sequence Data; Multigene Family; Myocardium/chemistry; Recombinant Proteins/analysis; Rosaniline Dyes; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0033554: cellular response to stress |
ECO:0000314: |
P |
Go to figure 1 |
complete | ||||
See also
References
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