GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9393726
| Citation |
Kotob, SI and Burns, DL (1997) Essential role of the consensus nucleotide-binding site of PtlH in secretion of pertussis toxin from Bordetella pertussis. J. Bacteriol. 179:7577-80 |
|---|---|
| Abstract |
PtlH is a member of a specialized set of transport proteins that is essential for secretion of pertussis toxin (PT) from Bordetella pertussis. Previously, PtlH was shown to contain a consensus nucleotide-binding motif. Here, we demonstrate that introduction of plasmids containing mutant forms of ptlH, altered in the putative nucleotide-binding region, into a wild-type strain of B. pertussis resulted in inhibition of PT secretion. Thus, this region of PtlH appears to be essential for protein function. Moreover, the observed dominant negative phenotype suggests that PtlH either functions as a multimer or interacts with another component necessary for secretion of PT. |
| Links | |
| Keywords |
Bacterial Proteins; Binding Sites; Biological Transport; Bordetella pertussis/genetics; Bordetella pertussis/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cloning, Molecular; Consensus Sequence; Membrane Transport Proteins; Mutagenesis, Site-Directed; Nucleotides/metabolism; Pertussis Toxin; Virulence Factors, Bordetella/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019534 : toxin transporter activity |
ECO:0000315: |
F |
Figure 5: you can compare the WT to the MT levels of pertussis toxin secretion. The MT cell line have a decreased level of toxin secretion showing that the gene is important for a functional transport system. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
