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PMID:9237995
| Citation |
Galinier, A, Haiech, J, Kilhoffer, MC, Jaquinod, M, Stülke, J, Deutscher, J and Martin-Verstraete, I (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc. Natl. Acad. Sci. U.S.A. 94:8439-44 |
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| Abstract |
Carbon catabolite repression (CCR) of several Bacillus subtilis catabolic genes is mediated by ATP-dependent phosphorylation of histidine-containing protein (HPr), a phosphocarrier protein of the phosphoenolpyruvate (PEP): sugar phosphotransferase system. In this study, we report the discovery of a new B. subtilis gene encoding a HPr-like protein, Crh (for catabolite repression HPr), composed of 85 amino acids. Crh exhibits 45% sequence identity with HPr, but the active site His-15 of HPr is replaced with a glutamine in Crh. Crh is therefore not phosphorylated by PEP and enzyme I, but is phosphorylated by ATP and the HPr kinase in the presence of fructose-1,6-bisphosphate. We determined Ser-46 as the site of phosphorylation in Crh by carrying out mass spectrometry with peptides obtained by tryptic digestion or CNBr cleavage. In a B. subtilis ptsH1 mutant strain, synthesis of beta-xylosidase, inositol dehydrogenase, and levanase was only partially relieved from CCR. Additional disruption of the crh gene caused almost complete relief from CCR. In a ptsH1 crh1 mutant, producing HPr and Crh in which Ser-46 is replaced with a nonphosphorylatable alanyl residue, expression of beta-xylosidase was also completely relieved from glucose repression. These results suggest that CCR of certain catabolic operons requires, in addition to CcpA, ATP-dependent phosphorylation of Crh, and HPr at Ser-46. |
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| Keywords |
Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Carbon/metabolism; Genes, Bacterial; Molecular Sequence Data; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Phosphoproteins/genetics; Phosphoproteins/metabolism; Phosphorylation |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009401: phosphoenolpyruvate-dependent sugar phosphotransferase system |
ECO:0000315: |
P |
Inositol dehydrogenase, beta-galactosidase, and beta-xylosidase activities were measured in the wild-type (QB5081), ptsH1 [QB5224 (34)], crh::aphA3, and ccpA::spc strains as well as in the double mutant ptsH1 crh::aphA3 under inducing or repressing (presence of fructose, glucose, or glycerol) conditions (Table 2). These results indicate that, in addition to CcpA, both HPr and Crh participate in CCR of certain genes in B. subtilis. |
complete | ||||
See also
References
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