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PMID:9182656

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Citation

Yang, L, Guan, T and Gerace, L (1997) Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis. J. Cell Biol. 137:1199-210

Abstract

We have analyzed the fate of several integral membrane proteins of the nuclear envelope during mitosis in cultured mammalian cells to determine whether nuclear membrane proteins are present in a vesicle population distinct from bulk ER membranes after mitotic nuclear envelope disassembly or are dispersed throughout the ER. Using immunofluorescence staining and confocal microscopy, we compared the localization of two inner nuclear membrane proteins (laminaassociated polypeptides 1 and 2 [LAP1 and LAP2]) and a nuclear pore membrane protein (gp210) to the distribution of bulk ER membranes, which was determined with lipid dyes (DiOC6 and R6) and polyclonal antibodies. We found that at the resolution of this technique, the three nuclear envelope markers become completely dispersed throughout ER membranes during mitosis. In agreement with these results, we detected LAP1 in most membranes containing ER markers by immunogold electron microscopy of metaphase cells. Together, these findings indicate that nuclear membranes lose their identity as a subcompartment of the ER during mitosis. We found that nuclear lamins begin to reassemble around chromosomes at the end of mitosis at the same time as LAP1 and LAP2 and propose that reassembly of the nuclear envelope at the end of mitosis involves sorting of integral membrane proteins to chromosome surfaces by binding interactions with lamins and chromatin.

Links

PubMed PMC2132536

Keywords

3T3 Cells; Animals; COS Cells; Cell Line; Cercopithecus aethiops; DNA-Binding Proteins; Endoplasmic Reticulum/metabolism; HeLa Cells; Humans; Intracellular Membranes; Lamins; Membrane Glycoproteins/metabolism; Membrane Proteins/metabolism; Mice; Microscopy, Confocal; Microscopy, Immunoelectron; Mitosis; Nuclear Envelope/metabolism; Nuclear Pore Complex Proteins; Nuclear Proteins/metabolism; Rabbits; Rats

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:PO210

colocalizes_with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:PO210

Colocalizes with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314:

C

Figure 3 shows the results from immunofluorescence assay.

complete
CACAO 10448

RAT:LRRC7

Colocalizes with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314:

C

Figure 2

complete
CACAO 10450

RAT:LRRC7

colocalizes_with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:LAP2

colocalizes_with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:LAP2

Colocalizes with

GO:0005789: endoplasmic reticulum membrane

ECO:0000314:

C

Figure 3

complete
CACAO 10451

Notes

See also

References

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