PMID:9150207

Citation	Colombi, D and Gomes, SL (1997) An alkB gene homolog is differentially transcribed during the Caulobacter crescentus cell cycle. J. Bacteriol. 179:3139-45
Abstract	A Caulobacter crescentus alkB gene homolog was identified in a clone previously shown to contain the heat shock genes dnaK and dnaJ; the homolog is located upstream of dnaK and is transcribed in the opposite orientation. An analysis of the alkB gene has shown that the deduced amino acid sequence is that of a 21-kDa protein, which is 42% identical and 78% similar to Escherichia coli AlkB. Furthermore, an alkB-null mutant was constructed by gene disruption and was shown to be highly sensitive to the alkylating agent methyl methanesulfonate (MMS). However, the alkB gene of C. crescentus, unlike its E. coli counterpart, is not located downstream of the ada gene, and its transcription is not induced by alkylating agents. In addition, no acquired enhanced resistance to MMS toxicity by treatment with low MMS doses was observed, suggesting that no adaptive response occurs in C. crescentus. Nevertheless, transcription of the alkB gene is cell cycle controlled, with a pattern of expression similar to that of several Caulobacter genes involved in DNA replication.
Links	PubMed PMC179090
Keywords	Adaptation, Physiological/drug effects; Alkylating Agents/pharmacology; Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/drug effects; Bacterial Proteins/genetics; Base Sequence; Caulobacter crescentus/cytology; Caulobacter crescentus/drug effects; Caulobacter crescentus/enzymology; Caulobacter crescentus/genetics; Cell Cycle/drug effects; Cell Cycle/genetics; Cytochrome P-450 CYP4A; Cytochrome P-450 Enzyme System/biosynthesis; Cytochrome P-450 Enzyme System/drug effects; Cytochrome P-450 Enzyme System/genetics; Gene Deletion; Gene Expression Regulation, Bacterial/drug effects; Genes, Bacterial/drug effects; Methyl Methanesulfonate/pharmacology; Mixed Function Oxygenases/biosynthesis; Mixed Function Oxygenases/drug effects; Mixed Function Oxygenases/genetics; Molecular Sequence Data; Mutation; Sequence Analysis, DNA; Transcription, Genetic/drug effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CAUCN:ALKB		GO:0006307: DNA dealkylation involved in DNA repair	ECO:0000315:		P		Figure 4 shows that the survival of Caulobacter crescentus with a deletion in the AlkB gene is compensated 100fold under .01% methyl methane sulfonate conditions. This shows that AlkB plays a role in the cells defense against alkylation.	complete CACAO 12728

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