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PMID:9023961
| Citation |
Rees, E, Siddiqui, RA, Köster, F, Schneider, B and Friedrich, B (1997) Structural gene (nirS) for the cytochrome cd1 nitrite reductase of Alcaligenes eutrophus H16. Appl. Environ. Microbiol. 63:800-2 |
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| Abstract |
Denitrification by Alcaligenes eutrophus H16 is genetically linked to megaplasmid pHG1. Unexpectedly, the gene encoding the nitrite reductase (nirS) was identified on chromosomal DNA. The nirS product showed extensive homology with periplasmic nitrite reductases of the heme cd1-type. Disruption of nirS abolished nitrite-reducing ability, indicating that NirS is the enzyme essential for denitrification in A.eutrophus. |
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| Keywords |
Alcaligenes/enzymology; Alcaligenes/genetics; Amino Acid Sequence; Chromosomes, Bacterial; Cytochromes; Electron Transport Complex IV/genetics; Genes, Bacterial; Molecular Sequence Data; Mutagenesis; Nitrite Reductases/genetics; Nitrites/metabolism; Oxidation-Reduction; Sequence Analysis, DNA; Sequence Homology, Amino Acid |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0050421: nitrite reductase (NO-forming) activity |
ECO:0000315: |
F |
Figure 3: The wild type and mutants were able to convert nitrate to nitrite. Nitrite added up in the medium and was not able to be reduced to nitrogen oxide. But, with the nirS it was able to. |
complete | ||||
|
enables |
GO:0050421: nitrite reductase (NO-forming) activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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