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PMID:8663104
| Citation |
Ng, JY and Marians, KJ (1996) The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes. J. Biol. Chem. 271:15642-8 |
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| Abstract |
The phiX-type primosome was discovered during the resolution and reconstitution in vitro of the complementary strand DNA replication step of the phiX174 viral life cycle. This multienzyme bidirectional helicase-primase complex can provide the DNA unwinding and Okazaki fragment-priming functions at the replication fork and has been implicated in cellular DNA replication, repair, and recombination. We have used gel mobility shift assays and enhanced chemiluminescence Western analysis to isolate and identify the pathway of primosome assembly at a primosome assembly site (PAS) on a 300-nucleotide-long single-stranded DNA fragment. The first three steps do not require ATP and are as follows: (i) PriA recognition and binding to the PAS, (ii) stabilization of the PriA-PAS complex by the addition of PriB, and (iii) formation of a PriA-PriB-DnaT-PAS complex. Subsequent formation of the preprimosome involves the ATP-dependent transfer of DnaB from a DnaB-DnaC complex to the PriA-PriB-DnaT-PAS complex. The final preprimosomal complex contains PriA, PriB, DnaT, and DnaB but not DnaC. A transient interaction between the preprimosome and DnaG generates the five-protein primosome. As described in an accompanying article (Ng, J. Y., and Marians, K. J. (1996) J. Biol. Chem. 271, 15649-15655), when assembled on intact phiX174 phage DNA, the primosome also contains PriC. |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; Bacterial Proteins/metabolism; Bacteriophage phi X 174/genetics; Bacteriophage phi X 174/ultrastructure; DNA Helicases; DNA Replication; DNA, Single-Stranded/metabolism; DNA, Viral/genetics; DNA, Viral/metabolism; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/metabolism; Deoxyribonucleoproteins/chemistry; DnaB Helicases; Escherichia coli/genetics; Escherichia coli Proteins; Macromolecular Substances; Replication Protein A |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0043234: protein complex |
ECO:0000021: |
UniProtKB:P0AEF0 UniProtKB:P0A8J2 UniProtKB:P0ACB0 UniProtKB:P17888
|
C |
Fig. 6 |
complete | |||
| GO:0043234: protein complex |
ECO:0000021: |
UniProtKB:P0AEF0 UniProtKB:P07013 UniProtKB:P0ACB0 UniProtKB:P17888
|
C |
Fig 6. |
complete | |||
| GO:0043234: protein complex |
ECO:0000021: |
UniProtKB:P0AEF0 UniProtKB:P07013 UniProtKB:P0A8J2 UniProtKB:P0ACB0
|
C |
Figure 6 |
complete | |||
| GO:0043234: protein complex |
ECO:0000021: |
UniProtKB:P0AEF0 UniProtKB:P07013 UniProtKB:P0A8J2 UniProtKB:P17888
|
C |
Fig. 6 |
complete | |||
| GO:0043234: protein complex |
ECO:0000021: |
UniProtKB:P0ACB0 UniProtKB:P07013 UniProtKB:P0A8J2 UniProtKB:P17888
|
C |
Fig. 6 |
complete | |||
See also
References
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