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PMID:8234269
| Citation |
Kibbey, MC, Jucker, M, Weeks, BS, Neve, RL, Van Nostrand, WE and Kleinman, HK (1993) beta-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin. Proc. Natl. Acad. Sci. U.S.A. 90:10150-3 |
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| Abstract |
We previously characterized a 110-kDa membrane-associated laminin-binding protein (LBP110) from brain which binds the laminin A chain -Ile-Lys-Val-Ala-Val-(IKVAV) site and increases in injury. Here we demonstrate that antisera directed against different epitopes of beta-amyloid precursor protein (APP) recognize LBP110 and that APP is recognized by LBP110 antiserum. APP specifically binds IKVAV and not another biologically active laminin-derived peptide containing the amino acid sequence -Tyr-Ile-Gly-Ser-Arg-. PC-12 cells transfected with antisense APP RNA produce less APP and LBP110, and they form fewer processes when cultured on either laminin or the IKVAV peptide. Thus, LBP110 is a member of the APP family and a function for APP in neurite outgrowth is now defined. |
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| Keywords |
Amino Acid Sequence; Amyloid beta-Protein Precursor/biosynthesis; Amyloid beta-Protein Precursor/metabolism; Animals; Binding Sites; Genetic Vectors; Laminin/chemistry; Laminin/metabolism; Molecular Sequence Data; Nerve Growth Factors/pharmacology; Neurites/physiology; PC12 Cells; Peptide Fragments/chemistry; Peptide Fragments/metabolism; RNA, Antisense; Transfection |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016322: transmembrane receptor protein tyrosine kinase activity |
ECO:0000314: |
F |
Figure 5: Neurite production by APP antisense cell lines - APP funciton in neurite outgrowth |
complete | ||||
See also
References
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