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PMID:8093698
| Citation |
Schreier, HJ, Rostkowski, CA and Kellner, EM (1993) Altered regulation of the glnRA operon in a Bacillus subtilis mutant that produces methionine sulfoximine-tolerant glutamine synthetase. J. Bacteriol. 175:892-7 |
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| Abstract |
A Bacillus subtilis mutant that produced glutamine synthetase (GS) with altered sensitivity to DL-methionine sulfoximine was isolated. The mutation, designated glnA33, was due to a T.A-to-C.G transition, changing valine to alanine at codon 190 within the active-site C domain. Altered regulation was observed for GS activity and antigen and mRNA levels in a B. subtilis glnA33 strain. The mutant enzyme was 28-fold less sensitive to DL-methionine sulfoximine and had a 13.0-fold-higher Km for hydroxylamine and a 4.8-fold-higher Km for glutamate than wild-type GS did. |
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| Keywords |
Bacillus subtilis/genetics; Genes, Bacterial; Glutamate-Ammonia Ligase/antagonists & inhibitors; Glutamate-Ammonia Ligase/genetics; Glutamates/metabolism; Kinetics; Methionine Sulfoximine/pharmacology; Nitrogen/metabolism; Operon; Restriction Mapping |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006542: glutamine biosynthetic process |
ECO:0000315: |
P |
The strain, named CAR2, did not exhibit detectable GS activity, and we could not detect any GS antigen when we probed [35S]methionine-labeled extracts with antibody to GS (Fig. 2). |
complete | ||||
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involved_in |
GO:0006542: glutamine biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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