PMID:7851795

Citation	Allshire, RC, Nimmo, ER, Ekwall, K, Javerzat, JP and Cranston, G (1995) Mutations derepressing silent centromeric domains in fission yeast disrupt chromosome segregation. Genes Dev. 9:218-33
Abstract	The ura4+ gene displays phenotypes consistent with variegated expression when inserted at 11 sites throughout fission yeast centromere 1. An abrupt transition occurs between the zone of centromeric repression and two adjacent expressed sites. Mutations in six genes alleviate repression of the silent-mating type loci and of ura4+ expressed from a site adjacent to the silent locus, mat3-M. Defects at all six loci affect repression of the ura4+ gene adjacent to telomeres and at the three centromeric sites tested. The clr4-S5 and rik1-304 mutations cause the most dramatic derepression at two out of three sites within cen1. All six mutations had only slight or intermediate effects on a third site in the center of cen1 or on telomeric repression. Strains with lesions at the clr4, rik1, and swi6 loci have highly elevated rates of chromosome loss. We propose that the products of these genes are integral in the assembly of a heterochromatin-like structure, with distinct domains, enclosing the entire centromeric region that reduces or excludes access to transcription factors. The formation of this heterochromatic structure may be an absolute requirement for the formation of a fully functional centromere.
Links	PubMed
Keywords	Base Sequence; Centromere/physiology; Chromosomes, Fungal/physiology; Crosses, Genetic; Gene Expression Regulation, Fungal/genetics; Genes, Fungal/genetics; Heterochromatin; Meiosis/physiology; Models, Genetic; Molecular Sequence Data; Mutation/physiology; RNA, Fungal/biosynthesis; RNA, Messenger/biosynthesis; Schizosaccharomyces/genetics; Schizosaccharomyces/metabolism; Telomere/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SCHPO:CLR4	involved_in	GO:0030702: chromatin silencing at centromere	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
SCHPO:SWI6	involved_in	GO:0006348: chromatin silencing at telomere	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
SCHPO:SWI6	involved_in	GO:0030702: chromatin silencing at centromere	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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