GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:7737119
| Citation |
Sandkvist, M, Bagdasarian, M, Howard, SP and DiRita, VJ (1995) Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J. 14:1664-73 |
|---|---|
| Abstract |
Vibrio cholerae secretes a number of proteins important for virulence, including cholera toxin. This process requires the products of the eps genes which have homologues in genera such as Aeromonas, Klebsiella and Pseudomonas and are thought to form a membrane-associated multiprotein complex. Here we show that the putative nucleotide-binding protein EpsE is associated with and stabilized by the cytoplasmic membrane via interaction with EpsL. Analysis of fusion proteins between EpsE and the homologous ExeE from Aeromonas hydrophila demonstrates that the N-terminus of EpsE contains the EpsL binding domain and determines species specificity. An intact Walker A box, commonly found in ATP-binding proteins, is required for activity of EpsE in vivo and for autophosphorylation of purified EpsE in vitro. These results indicate that both the kinase activity of EpsE as well as its ability to interact with the putative cytoplasmic membrane protein EpsL are required for translocation of toxin across the outer membrane in Vibrio cholerae. |
| Links | |
| Keywords |
Adenosine Triphosphate/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Bacterial Proteins/secretion; Base Sequence; Cell Compartmentation; Cell Membrane/enzymology; Cell Membrane/metabolism; Cholera Toxin/metabolism; Genetic Complementation Test; Membrane Proteins/genetics; Molecular Sequence Data; Phosphorylation; Protein Binding; Protein Conformation; Protein Kinases/metabolism; Sequence Homology, Amino Acid; Species Specificity; Structure-Activity Relationship; Vibrio cholerae/enzymology; Vibrio cholerae/genetics; Vibrio cholerae/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031235: intrinsic to internal side of plasma membrane |
ECO:0000315: |
C |
Figure 3: You can see that the protein localizes in the plasma membrane. |
complete | ||||
| GO:0019534: toxin transporter activity |
ECO:0000315: |
F |
Table 1: the mutant type secretes less toxin. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
