PMID:7642319

Citation	McKee, ML, Melton-Celsa, AR, Moxley, RA, Francis, DH and O'Brien, AD (1995) Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect. Immun. 63:3739-44
Abstract	In a previous study, enterohemorrhagic Escherichia coli (EHEC) O157:H7 with a deletion and insertion in the eaeA gene encoding intimin was used to establish that intimin is required for the organism to attach to and efface microvilli in the piglet intestine (M. S. Donnenberg, S. Tzipori, M. L. McKee, A. D. O'Brien, J. Alroy, and J. B. Kaper, J. Clin. Invest. 92:1418-1424, 1993). However, in the same investigation, a role for intimin in EHEC adherence to HEp-2 cells could not be definitively demonstrated. To analyze the basis for this discrepancy, we constructed an in-frame deletion of eaeA and compared the adherence capacity of this mutant with that of the wild-type strain in vitro and in vivo. We observed a direct correlation between the requisite for intimin in EHEC O157:H7 colonization of the gnotobiotic piglet intestine and adherence of the bacterium to HEp-2 cells. The in vitro-in vivo correlation lends credence to the use of the HEp-2 cell adherence model for further study of the intimin protein.
Links	PubMed PMC173523
Keywords	Adhesins, Bacterial; Animals; Bacterial Adhesion; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/physiology; Base Sequence; Carrier Proteins; Cell Line; Escherichia coli/pathogenicity; Escherichia coli Proteins; Germ-Free Life; Intestines/microbiology; Molecular Sequence Data; Swine
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECO57:EAE		GO:0009405: pathogenesis	ECO:0000315:		P		Table 1. shows that eaeA negative strains were nonadherent in vitro to HEp-2 cells and unable to cause A/E lesions.	complete CACAO 6020

References

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