PMID:7595232

Citation	Airas, L, Hellman, J, Salmi, M, Bono, P, Puurunen, T, Smith, DJ and Jalkanen, S (1995) CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73. J. Exp. Med. 182:1603-8
Abstract	We have recently described a monoclonal antibody (mAb) 4G4 recognizing a 70-kD molecule constitutively expressed on human endothelial cells and on subpopulations of lymphocytes. We showed that this molecule, which we named lymphocyte-vascular adhesion protein 2 (L-VAP-2), mediates lymphocyte adhesion to cultured endothelial cells. Protein sequencing of tryptic peptides from immunoaffinity-purified L-VAP-2 revealed sequence identity between L-VAP-2 and CD73 (ecto-5'-nucleotidase, E.C.3.1.3.5), and COS cells transfected with a CD73 cDNA were positively stained with the mAb 4G4, which recognizes L-VAP-2. mAb 4G4 was also able to partially inhibit the ecto-5'-nucleotidase activity of peripheral blood lymphocytes. Moreover, cross-precipitation studies performed with mAb 4G4 and a CD73 workshop mAb 1E9 showed that these two antibodies recognize the same molecule. Since the tissue distribution and biochemical characteristics of the two molecules are also similar, we conclude that L-VAP-2 and CD73 are the same glycoprotein. Adhesion experiments showed significantly increased binding of freshly isolated lymphocytes to COS cells transfected with a CD73 cDNA, as compared to mock-transfected COS cells, and binding of lymphocytes to CD73-expressing COS cells was inhibited by the presence of mAb 4G4 in the adhesion assay. CD73 is a glycosyl phosphatidylinositol-linked molecule previously shown to have a cosignalling role in T lymphocyte proliferation. Our data suggest that it also has a function in mediating lymphocyte adhesion to the endothelium.
Links	PubMed PMC2192217
Keywords	5'-Nucleotidase/genetics; 5'-Nucleotidase/immunology; 5'-Nucleotidase/physiology; Amino Acid Sequence; Animals; Antibodies, Monoclonal/immunology; Base Sequence; Cell Adhesion; Cell Adhesion Molecules/genetics; Cell Adhesion Molecules/immunology; Cell Adhesion Molecules/physiology; Cell Line, Transformed; Cercopithecus aethiops; DNA, Complementary/genetics; Endothelium, Vascular/cytology; Humans; Lymphocytes/cytology; Molecular Sequence Data; Sequence Homology, Amino Acid; Transfection
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:5NTD		GO:0007159: leukocyte cell-cell adhesion	ECO:0000314:		P		Fig 5 clearly shows that CD73 is involved in lymphocyte binding to COS cells	complete CACAO 5606
HUMAN:5NTD		GO:0008253: 5'-nucleotidase activity	ECO:0000314:		F		Fig. 4 shows inhibition of ecto-5'-NT activity via different anti- CD73 mAbs.	complete CACAO 5819
HUMAN:5NTD	enables	GO:0008253: 5'-nucleotidase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:5NTD	involved_in	GO:0007159: leukocyte cell-cell adhesion	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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