PMID:7565692

Citation	Chen, J, Tomkinson, AE, Ramos, W, Mackey, ZB, Danehower, S, Walter, CA, Schultz, RA, Besterman, JM and Husain, I (1995) Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination. Mol. Cell. Biol. 15:5412-22
Abstract	Three biochemically distinct DNA ligase activities have been identified in mammalian cell extracts. We have recently purified DNA ligase II and DNA ligase III to near homogeneity from bovine liver and testis tissue, respectively. Amino acid sequencing studies indicated that these enzymes are encoded by the same gene. In the present study, human and murine cDNA clones encoding DNA ligase III were isolated with probes based on the peptide sequences. The human DNA ligase III cDNA encodes a polypeptide of 862 amino acids, whose sequence is more closely related to those of the DNA ligases encoded by poxviruses than to replicative DNA ligases, such as human DNA ligase I. In vitro transcription and translation of the cDNA produced a catalytically active DNA ligase similar in size and substrate specificity to the purified bovine enzyme. The DNA ligase III gene was localized to human chromosome 17, which eliminated this gene as a candidate for the cancer-prone disease Bloom syndrome that is associated with DNA joining abnormalities. DNA ligase III is ubiquitously expressed at low levels, except in the testes, in which the steady-state levels of DNA ligase III mRNA are at least 10-fold higher than those detected in other tissues and cells. Since DNA ligase I mRNA is also present at high levels in the testes, we examined the expression of the DNA ligase genes during spermatogenesis. DNA ligase I mRNA expression correlated with the contribution of proliferating spermatogonia cells to the testes, in agreement with the previously defined role of this enzyme in DNA replication. In contrast, elevated levels of DNA ligase III mRNA were observed in primary spermatocytes undergoing recombination prior to the first meiotic division. Therefore, we suggest that DNA ligase III seals DNA strand breaks that arise during the process of meiotic recombination in germ cells and as a consequence of DNA damage in somatic cells.
Links	PubMed PMC230791
Keywords	Amino Acid Sequence; Animals; Base Sequence; Cattle; Chromosome Mapping; Chromosomes, Human, Pair 17; Cloning, Molecular; DNA Damage; DNA Ligases/biosynthesis; DNA Ligases/genetics; DNA Ligases/metabolism; Gene Expression Regulation, Developmental; Humans; Male; Meiosis/genetics; Mice; Molecular Sequence Data; Organ Specificity; RNA, Messenger/analysis; Recombination, Genetic/physiology; Sequence Alignment; Spermatocytes/enzymology; Substrate Specificity; Testis/enzymology; Testis/growth & development
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:DNLI3	enables	GO:0003910: DNA ligase (ATP) activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:DNLI3	involved_in	GO:0006266: DNA ligation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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