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PMID:3569533

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Citation

Hammond, GL, Underhill, DA, Smith, CL, Goping, IS, Harley, MJ, Musto, NA, Cheng, CY and Bardin, CW (1987) The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain. FEBS Lett. 215:100-4

Abstract

We have sequenced a cDNA for sex hormone-binding globulin (SHBG) isolated from a phage lambda gt11 human liver cDNA library. The library was screened with a radiolabeled rat androgen-binding protein (ABP) cDNA, and the abundance of SHBG cDNAs was 1 in 750,000 plaques examined. The largest human SHBG cDNA (1194 base-pairs) contained a reading frame for 381 amino acids. This comprised 8 amino acids of a signal peptide followed by 373 residues starting with the known NH2-terminal sequence of human SHBG, and ending with a termination codon. The predicted polypeptide Mr of SHBG is 40,509, and sites of attachment of one O-linked (residue 7) and two N-linked oligosaccharide (residues 351 and 367) chains were identified. Purified SHBG was photoaffinity-labeled with delta 6-[3H]testosterone and cleaved with trypsin. The labeled tryptic fragment was isolated by reverse-phase HPLC, and its NH2-terminal sequence was determined. The results suggest that a portion of the steroid-binding domain of SHBG is located between residue 296 and the 35 predominantly hydrophilic residues at the C-terminus of the protein.

Links

PubMed

Keywords

Amino Acid Sequence; Base Sequence; Binding Sites; DNA/analysis; DNA, Recombinant/analysis; Gonadal Steroid Hormones/metabolism; Humans; Male; Protein Binding; Sex Hormone-Binding Globulin/genetics; Sex Hormone-Binding Globulin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SHBG

GO:0007285: primary spermatocyte growth

ECO:0000255:

PMID:3569533[1]

P

See Figure 2. It shows the amino acid sequence of SHBG gotten from the sequence of its cDNA.

complete
CACAO 9352

See also

References

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  1. Hammond, GL et al. (1987) The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain. FEBS Lett. 215 100-4 PubMed GONUTS page