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PMID:3092189
| Citation |
Gribskov, M and Burgess, RR (1986) Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res. 14:6745-63 |
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| Abstract |
We show, using dot matrix comparisons and statistical analysis of sequence alignments, that seven sequenced sigma factors, E. coli sigma-70 and sigma-32, B. subtilis sigma-43 and sigma-29, phage SP01 gene products 28 and 34, and phage T4 gene product 55, comprise a homologous family of proteins. Sigma-70, sigma-32, and sigma-43 each have two copies of a sequence similar to the helix-turn-helix DNA binding motif seen in CRP, and lambda repressor and cro proteins. B. subtilis sigma-29, SP01 gp28, and SP01 gp34 have at least one copy similar to this sequence. We propose that a second sequence, conserved in all seven proteins is the core RNA polymerase binding site. A third region, present only in sigma-70 and sigma-43, may also be involved in interaction with core. Available mutational evidence supports our model for sigma factor structure. |
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| Keywords |
Amino Acid Sequence; Bacillus subtilis/genetics; Bacteriophages/genetics; Binding Sites; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Mutation; Protein Binding; Sigma Factor/genetics; Species Specificity; T-Phages/genetics; Transcription Factors/genetics |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003677: DNA binding |
ECO:0000314: |
F |
Sequence alignments of the sigma70 family members reveal four conserved regions that can be further divided into subregions eg. sub-region 2.2, which may be involved in the binding of the sigma factor to the core RNA polymerase |
complete | ||||
Notes
See also
References
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