PMID:30804227

Citation	Albert, B, Tomassetti, S, Gloor, Y, Dilg, D, Mattarocci, S, Kubik, S, Hafner, L and Shore, D (2019) Sfp1 regulates transcriptional networks driving cell growth and division through multiple promoter-binding modes. Genes Dev. 33:288-293
Abstract	The yeast Sfp1 protein regulates both cell division and growth but how it coordinates these processes is poorly understood. We demonstrate that Sfp1 directly controls genes required for ribosome production and many other growth-promoting processes. Remarkably, the complete set of Sfp1 target genes is revealed only by a combination of ChIP (chromatin immunoprecipitation) and ChEC (chromatin endogenous cleavage) methods, which uncover two promoter binding modes, one requiring a cofactor and the other a DNA-recognition motif. Glucose-regulated Sfp1 binding at cell cycle "START" genes suggests that Sfp1 controls cell size by coordinating expression of genes implicated in mass accumulation and cell division.
Links	PubMed PMC6411004 Online version:10.1101/gad.322040.118
Keywords	Chromatin Immunoprecipitation; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Gene Expression Regulation, Fungal/genetics; Gene Regulatory Networks/genetics; Glucose/metabolism; Promoter Regions, Genetic/genetics; Protein Binding; RNA Polymerase II/metabolism; Regulon/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:SFP1		GO:0003676: nucleic acid binding	ECO:0000256: match to sequence model evidence used in automatic assertion		F		Figure 1. Regulation of growth-related genes by Sfp1 (temporary annotation)	complete

References

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