PMID:2971041

Citation	Deak, SB, Pierce, RA, Belsky, SA, Riley, DJ and Boyd, CD (1988) Rat tropoelastin is synthesized from a 3.5-kilobase mRNA. J. Biol. Chem. 263:13504-7
Abstract	A lambda gt11 cDNA library was constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats and screened with a human tropoelastin cDNA clone. DNA sequence analysis of several overlapping rat clones confirmed the presence of DNA sequences coding for murine tropoelastin and DNA sequences coding for the 3'-untranslated region of the rat tropoelastin mRNA. Northern blot analysis of total RNA from aortic tissue of neonatal rats using oligonucleotide probes derived from these rat tropoelastin cDNAs demonstrated the presence of a 3.5-kilobase tropoelastin mRNA. The size of this rat tropoelastin mRNA agrees with previous reports for the size of the mRNA coding for tropoelastin in tissue from several vertebrate species but contrasts with several reports suggesting the presence of a higher molecular weight mRNA species responsible for the synthesis of tropoelastin in rodent tissue.
Links	PubMed
Keywords	Animals; Animals, Newborn; Aorta/analysis; Bacteriophage lambda/genetics; Base Sequence; DNA/genetics; DNA, Recombinant; Elastin/analogs & derivatives; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Protein Biosynthesis; RNA, Messenger/genetics; Rats; Sequence Homology, Nucleic Acid; Transcription, Genetic; Tropoelastin/biosynthesis; Tropoelastin/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:ELN		GO:0005739: mitochondrion	ECO:0000314:		C		Figure 5 shows a northern blot analysis of the RNA isolated from Rat. Panel showing the total RNA isolated from aortic tissue	complete

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