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PMID:2971020
| Citation |
Russell, RR, Mukasa, H, Shimamura, A and Ferretti, JJ (1988) Streptococcus mutans gtfA gene specifies sucrose phosphorylase. Infect. Immun. 56:2763-5 |
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| Abstract |
The ethanol-insoluble product formed from sucrose by purified enzyme encoded by the gtfA gene from Streptococcus mutans, expressed in recombinant Escherichia coli, was analyzed by 13C and 31P nuclear magnetic resonance. The product was identified as alpha-D-glucose-1-phosphate, and it was concluded that the GTF-A enzyme is sucrose phosphorylase (sucrose:orthophosphate alpha-D-glucosyltransferase [EC 2.4.1.7]). |
| Links | |
| Keywords |
DNA, Bacterial/genetics; Genes, Bacterial; Glucosyltransferases/genetics; Magnetic Resonance Spectroscopy; Streptococcus mutans/genetics; Sucrose/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005985: sucrose metabolic process |
ECO:0000314: |
P |
Figure 3 |
complete | ||||
| GO:0009018: sucrose phosphorylase activity |
ECO:0000314: |
F |
Figures 1 and 2 show that a product of GtfA reacting with sucrose and phosphate is glucose-1-phosphate, and figure 3 shows the reaction also yields fructose, thus sucrose phosphorylase activity is shown. |
complete | ||||
|
enables |
GO:0009018: sucrose phosphorylase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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