PMID:29666193

Citation	David, DJ, Pagliuso, A, Radoshevich, L, Nahori, MA and Cossart, P' (2018) Lmo1656 is a secreted virulence factor of Listeria monocytogenes that interacts with the sortin nexin 6-BAR complex. J. Biol. Chem. '
Abstract	Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components.  Here, we investigated a putatively secreted protein of Lm, Lmo1656, that is present in most sequenced strains of Lm, but is absent in the non-pathogenic species Listeria innocua. Lmo1656 gene is predicted to encode a small, positively-charged protein.  We show that Lmo1656 is secreted by Lm.  Furthermore, deletion of the lmo1656 gene (Δlmo1656) attenuates virulence in mice infected orally but not intravenously, suggesting that lmo1656 plays a role during oral listeriosis. We identified sorting nexin 6 (SNX6), an endosomal sorting component and BAR-domain containing protein, as a host cell interactor of Lmol656. SNX6 colocalizes with wild-type Lm during the early steps of infection. This colocalization depends on Lmo1656 and RNAi of SNX6 impairs infection in infected tissue culture cells, suggesting that SNX6 is utilized by Lm during infection. Our results reveal that Lmo1656 is a novel secreted virulence factor of Lm that facilitates recruitment of a specific member -of the sorting nexin family in the mammalian host.
Links	PubMed Online version:10.1074/jbc.RA117.000365
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LISMO:Q8Y6M8		GO:0009405: pathogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		The protein Lmo1656 is a virulence factor secreted by Listeria Monocytogenes. In figure 3 C -D orally innoculated mice infected with the mutant version delta lmo1656 were found to have lower bacterial burden 48 hours post infection. The proposed mechanism is through lm protein docking at E-CAD surface protein. They have also done the same test intravenously and it shows no decrease in virulence suggesting that this protein specifically helps the bacteria when infecting through the oral route.	complete CACAO 13294

References

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