PMID:28751710

Citation	Shrestha, RL, Conti, D, Tamura, N, Braun, D, Ramalingam, RA, Cieslinski, K, Ries, J and Draviam, VM (2017) Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells. Nat Commun 8:150
Abstract	Human chromosomes are captured along microtubule walls (lateral attachment) and then tethered to microtubule-ends (end-on attachment) through a multi-step end-on conversion process. Upstream regulators that orchestrate this remarkable change in the plane of kinetochore-microtubule attachment in human cells are not known. By tracking kinetochore movements and using kinetochore markers specific to attachment status, we reveal a spatially defined role for Aurora-B kinase in retarding the end-on conversion process. To understand how Aurora-B activity is counteracted, we compare the roles of two outer-kinetochore bound phosphatases and find that BubR1-associated PP2A, unlike KNL1-associated PP1, plays a significant role in end-on conversion. Finally, we uncover a novel role for Aurora-B regulated Astrin-SKAP complex in ensuring the correct plane of kinetochore-microtubule attachment. Thus, we identify Aurora-B as a key upstream regulator of end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process.Human chromosomes are captured along microtubule walls and then tethered to microtubule-ends through a multi-step end-on conversion process. Here the authors show that Aurora-B regulates end-on conversion in human cells and establish a late role for Astrin-SKAP complex in the end-on conversion process.
Links	PubMed PMC5532248 Online version:10.1038/s41467-017-00209-z
Keywords	Aurora Kinase B/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; HeLa Cells; Humans; Immunoblotting; Kinetochores/metabolism; Microscopy, Fluorescence; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Phosphoric Monoester Hydrolases/metabolism; Protein Binding; RNA Interference; Signal Transduction; Time-Lapse Imaging/methods
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:AURKB		GO:0004674: protein serine/threonine kinase activity	ECO:0000314:		F		Fig. 1 shows that in human cells, Aurora-B (Aurora Kinase B, AURKB) is responsible for phosphorylation of serine residues on the kinetochore HEC1 protein.	complete CACAO 13489
HUMAN:AURKB		GO:1905116: positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore	ECO:0000314:		P		Fig. 1 shows that in human cells, Aurora-B (Aurora Kinase B, AURKB) is very active on lateral kinetochores and that this activity promotes association of kinetochores and microtubules.	complete CACAO 13482

Notes

Scavenger Hunt - 3

References

See Help:References for how to manage references in GONUTS.