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PMID:28735115

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Citation

Cui, X, Yaghmaiean, H, Wu, G, Wu, X, Chen, X, Thorn, G and Wang, A (2017) The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex. Virology 510:147-155

Abstract

Like other positive-strand RNA viruses, plant potyviruses assemble viral replication complexes (VRCs) on modified cellular membranes. Potyviruses encode two membrane proteins, 6K2 and P3. The former is known to play pivotal roles in the formation of membrane-associated VRCs. However, P3 remains to be one of the least characterized potyviral proteins. The P3 cistron codes for P3 as well as P3N-PIPO which results from RNA polymerase slippage. In this study, we show that the P3N-PIPO of Turnip mosaic virus (TuMV) is required for viral cell-to-cell movement but not for viral replication. We demonstrate that the C-terminal region of P3 (P3C) is indispensable for P3 to form cytoplasmic punctate inclusions and target VRCs. We reveal that TuMV mutants that lack P3C are replication-defective. Taken together, these data suggest that the P3 cistron has two distinct functions: P3N-PIPO as a dedicated movement protein and P3 as an essential component of the VRC.

Links

PubMed Online version:10.1016/j.virol.2017.07.016

Keywords

DNA Mutational Analysis; Membrane Proteins/genetics; Membrane Proteins/metabolism; Plant Viral Movement Proteins/genetics; Plant Viral Movement Proteins/metabolism; Potyvirus/physiology; Tobacco; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Release; Virus Replication

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

TUMVJ:MVP

GO:0046740: transport of virus in host, cell to cell

ECO:0000315:

P

Organism: Turnip mosaic virus (TuMV) protein is PIPO and is referred to as P3N-PIPO polyprotein in Uniprot. The mutant without PIPO was unable to move. "Confocal microscopy revealed that the PIPO knock-out mutant TuMV-GFP(∆PIPO) was contained in a single cell and lost the ability to move to the neighboring cells 4 days post agroinfiltration (dpa) (Fig. 1B)."Figure 1B clearly shows that transport of the virus from cell to cell is stopped by loss of P3 meaning that P3 is necessary for transport of virus in host from cell to cell. Host: Nicotiana benthamiana

complete
CACAO 12987

TUMVJ:POLG

GO:0009405: pathogenesis

ECO:0000315:

P

organism: Turnip mosaic virus Protein is P3 in the paper. P3 in polyprotein POLG (Uniprot)."TuMV-GFP showed typical mosaic and leaf curling symptoms in upper new leaves, while the corresponding leaves in those inoculated by TuMV-GFP(∆GDD) and TuMV-GFP(∆P3C) did not display any symptoms (Fig. 2B)." Host: N. benthamiana

complete
CACAO 13046

TUMVJ:POLG

GO:0039690: positive stranded viral RNA replication

ECO:0000315:

P

The organism is Turnip mosaic virus and the protein is P3 in the paper. P3 in polyprotein POLG (Uniprot). Fig 3 shows that the delta P3 is deficient in positive stranded RNA viral replication after 24 hours compared to the wild type virus "The P3C deletion mutant showed slightly higher levels of (+) and (−) viral RNAs than the replication-defective mutant TuMV-GFP(∆GDD) at both time points (Fig. 3) with a significant difference (P<0.05) only found between the (+) viral RNA levels of the P3C deletion"

complete
CACAO 13047

Notes

See also

References

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