PMID:28406067

Citation	Nakase, J, Ukawa, Y, Takemoto, S, Kubo, T, Sagesaka, YM, Aoki-Yoshida, A and Totsuka, M' (2017) RNA and a cell wall component of Enterococcus faecalis IC-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line J774.1. Biosci. Biotechnol. Biochem. ':1-7
Abstract	Enterococcus faecalis is a resident lactic acid bacterium in the human intestine. Its immunostimulatory action was reported to be enhanced by heat sterilization. To investigate its beneficial actions, we evaluated the ability of 10 E. faecalis strains to induce interleukin-12 (IL-12) production in a mouse macrophage cell line, J774.1 and found that the strain, E. faecalis IC-1, had a potent IL-12-inducing ability. Furthermore, we investigated the underlying mechanism by treating IC-1 cells with RNase or lysozyme. Its activity almost disappeared and an antagonist of Toll-like receptor (TLR) 7 inhibited this activity. Moreover, lysozyme-treated IC-1 bacteria were not phagocytized by J774.1 cells, and did not induce IL-12 production. Based on our results, we propose that macrophages recognize the cell wall components of IC-1, leading to phagocytosis. The IC-1 RNA is then recognized by TLR7, which induces the production of IL-12.
Links	PubMed Online version:10.1080/09168451.2017.1295799
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Significance

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:ANG4		GO:0009986: cell surface	ECO:0000314:		C		lysozyme-treated bacteria were bound to the surface of the J774.1 cells, but were not phagocytized. Thus, RNA, the active ingredient of IC-1 cells, was not transferred into the J774.1 cells or recognized by the intracellular TLR7. Consequently, it failed to induce IL-12 production. By author, Fig. 6 Protein: Angiogenin-4 Organism: Mouse	complete CACAO 12693

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