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PMID:28337542
| Citation |
'Paillusson, S, Gomez-Suaga, P, Stoica, R, Little, D, Gissen, P, Devine, MJ, Noble, W, Hanger, DP and Miller, CC (2017) α-Synuclein binds to the ER-mitochondria tethering protein VAPB to disrupt Ca(2+) homeostasis and mitochondrial ATP production. Acta Neuropathol. ' |
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| Abstract |
α-Synuclein is strongly linked to Parkinson's disease but the molecular targets for its toxicity are not fully clear. However, many neuronal functions damaged in Parkinson's disease are regulated by signalling between the endoplasmic reticulum (ER) and mitochondria. This signalling involves close physical associations between the two organelles that are mediated by binding of the integral ER protein vesicle-associated membrane protein-associated protein B (VAPB) to the outer mitochondrial membrane protein, protein tyrosine phosphatase-interacting protein 51 (PTPIP51). VAPB and PTPIP51 thus act as a scaffold to tether the two organelles. Here we show that α-synuclein binds to VAPB and that overexpression of wild-type and familial Parkinson's disease mutant α-synuclein disrupt the VAPB-PTPIP51 tethers to loosen ER-mitochondria associations. This disruption to the VAPB-PTPIP51 tethers is also seen in neurons derived from induced pluripotent stem cells from familial Parkinson's disease patients harbouring pathogenic triplication of the α-synuclein gene. We also show that the α-synuclein induced loosening of ER-mitochondria contacts is accompanied by disruption to Ca(2+) exchange between the two organelles and mitochondrial ATP production. Such disruptions are likely to be particularly damaging to neurons that are heavily dependent on correct Ca(2+) signaling and ATP. |
| Links |
PubMed Online version:10.1007/s00401-017-1704-z |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:2001170: negative regulation of ATP biosynthetic process |
ECO:0000314: |
P |
Expression of alpha-synuclein, as seen in Figure 7, shows reduced mitochondrial ATP production in rat cortical neurons. Alpha-synuclein Rat |
complete | ||||
| GO:0032865: ERMES complex |
ECO:0000314: |
C |
Alpha-Synuclein is a MAM protein (Mitochondria associated ER membranes) and can be seen as such in Figure 8A. Alpha-synuclein Rat |
complete | ||||
Notes
See also
References
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