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PMID:28290554
| Citation |
Riedel, C, Lamp, B, Hagen, B, Indik, S and Rümenapf, T (2017) The core protein of a pestivirus protects the incoming virus against IFN-induced effectors. Sci Rep 7:44459 |
|---|---|
| Abstract |
A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors - have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately after entry. In this study, we take advantage of the previously reported property of Classical swine fever virus (family Flaviviridae, genus Pestivirus) to tolerate a deletion of the core protein if a compensatory mutation is present in the NS3-helicase-domain (Vp447). In contrast to the parental virus (Vp447), which causes a hemorrhagic-fever-like disease in pigs, Vp447 is avirulent in vivo. In comparison to Vp447, growth of Vp447 in primary porcine cells and IFN-treated porcine cell lines was reduced >20-fold. Also, primary porcine endothelial cells and IFN-pretreated porcine cell lines were 8-24 times less susceptible to Vp447. This reduction of susceptibility could be partially reversed by loading Vp447 particles with different levels of core protein. In contrast, expression of core protein in the recipient cell did not have any beneficial effect. Therefore, a protective effect of core protein in the incoming virus particle against the products of IFN-stimulated genes could be demonstrated. |
| Links |
PubMed PMC5349576 Online version:10.1038/srep44459 |
| Keywords |
Animals; Classical Swine Fever Virus/genetics; Classical Swine Fever Virus/pathogenicity; DNA Viruses/genetics; Pestivirus/genetics; Pestivirus/pathogenicity; Pestivirus Infections/genetics; Pestivirus Infections/virology; Swine/virology; Viral Core Proteins/genetics; Virus Replication/genetics |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0039502: suppression by virus of host type I interferon-mediated signaling pathway |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
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Notes
See also
References
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