PMID:28163713

Citation	Byun, MY, Cui, LH, Oh, TK, Jung, YJ, Lee, A, Park, KY, Kang, BG and Kim, WT (2017) Homologous U-box E3 Ubiquitin Ligases OsPUB2 and OsPUB3 Are Involved in the Positive Regulation of Low Temperature Stress Response in Rice (Oryza sativa L.). Front Plant Sci 8:16
Abstract	Rice U-box E3 Ub ligases (OsPUBs) are implicated in biotic stress responses. However, their cellular roles in response to abiotic stress are poorly understood. In this study, we performed functional analyses of two homologous OsPUB2 and OsPUB3 in response to cold stress (4°C). OsPUB2 was up-regulated by high salinity, drought, and cold, whereas OsPUB3 was constitutively expressed. A subcellular localization assay revealed that OsPUB2 and OsPUB3 were localized to the exocyst positive organelle (EXPO)-like punctate structures. OsPUB2 was also localized to the nuclei. OsPUB2 and OsPUB3 formed a hetero-dimeric complex as well as homo-dimers in yeast cells and in vitro. OsPUB2/OsPUB3 exhibited self-ubiquitination activities in vitro and were rapidly degraded in the cell-free extracts with apparent half-lives of 150-160 min. This rapid degradation of OsPUB2/OsPUB3 was delayed in the presence of the crude extracts of cold-treated seedlings (apparent half-lives of 200-280 min). Moreover, a hetero-dimeric form of OsPUB2/OsPUB3 was more stable than the homo-dimers. These results suggested that OsPUB2 and OsPUB3 function coordinately in response to cold stress. OsPUB2- and OsPUB3-overexpressing transgenic rice plants showed markedly better tolerance to cold stress than did the wild-type plants in terms of survival rates, chlorophyll content, ion leakage, and expression levels of cold stress-inducible marker genes. Taken together, these results suggested that the two homologous rice U-box E3 Ub ligases OsPUB2 and OsPUB3 are positive regulators of the response to cold stress.
Links	PubMed PMC5247461 Online version:10.3389/fpls.2017.00016
Keywords
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ORYSJ:Q75GN8		GO:0051865: protein autoubiquitination	ECO:0000315:		P		Organism Oryza sativa subsp. japonica (Rice), RING-type E3 ubiquitin transferase, OSPub2 (XP_015637531) "Myc-OsPUB2 and a single amino acid substitution mutant mutant Myc-OsPUB2C281A proteins were used for in vitro self-ubiquitination assay by using anti-Myc and anti-Ub antibodies” by author. In Fig. 2	complete CACAO 12301
ORYSJ:Q8LRC7	Colocalizes with	GO:0000145: exocyst	ECO:0000314:		C		Oryza sativa subsp. japonica (Rice), Arm repeat protein, known as osPUB3 (XP_015621922) in the paper was viewed through Fluorescence microscopy, which showed concentration of protein in the exocyst of the protoplasts in 2nd row of Fig. 3A.	complete CACAO 12337
ORYSJ:Q8LRC7		GO:0051865: protein autoubiquitination	ECO:0000315:		P		Organism Oryza sativa subsp. japonica (Rice), Arm repeat protein, "Myc-OsPUB3 (xp 015621922) and a single amino acid substitution mutant Myc-OsPUB3C280A proteins were used for in vitro self-ubiquitination assay by using anti-Myc and anti-Ub antibodies” by author. In Fig. 2	complete CACAO 12338

References

See Help:References for how to manage references in GONUTS.