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PMID:27718188
| Citation |
Valachovič, M and Hapala, I (2017) Biosynthetic Approaches to Squalene Production: The Case of Yeast. Methods Mol. Biol. 1494:95-106 |
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| Abstract |
Squalene is a precursor in the eukaryotic sterol biosynthesis. It is a valuable compound with several human health-related applications. Since the traditional natural resources of squalene are limited, alternatives for the production of squalene on industrial scale have been intensively explored during past years. The yeast Saccharomyces cerevisiae represents an attractive option due to elaborated techniques of genetic and metabolic engineering that can be applied to improve squalene yields. We discuss in this chapter some theoretical aspects of genetic manipulations of the ergosterol biosynthesis pathway aimed at increased squalene production and describe analytical methods for squalene purification and determination of its content in yeast cells. |
| Links |
PubMed Online version:10.1007/978-1-4939-6445-1_7 |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004506: squalene monooxygenase activity |
ECO:0000315: |
F |
Figure 2 in the paper shows the levels of ergosterol and squalene in two types of Saccharomyces cerevisiae. The first is a wild type organism with the gene for erg1^L37P operational. The second is a spontaneous mutant with a change in the erg1^L37P. The figure shows a chromatogram that shows lipid extracts with the levels of ergosterol and squalene shown. It is clear that the wild type shown has a high levels of ergosterol, representing normal function. The wild type shown to the right (with the erg1^L37P mutated) shows low levels of ergosterol and high levels of squalene. Assuming that no other gene was mutated or changed, this shows that squalene is an intermediate in the production of ergosterol. |
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Notes
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References
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