PMID:27467129

Citation	Charles Richard, JL, Shukla, MS, Menoni, H, Ouararhni, K, Lone, IN, Roulland, Y, Papin, C, Ben Simon, E, Kundu, T, Hamiche, A, Angelov, D and Dimitrov, S (2016) FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. PLoS Genet. 12:e1006221
Abstract	FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.
Links	PubMed PMC4965029 Online version:10.1371/journal.pgen.1006221
Keywords	Animals; Chromatin/genetics; Chromatin Assembly and Disassembly/genetics; Chromosomal Proteins, Non-Histone/genetics; DNA Damage/genetics; DNA Repair/genetics; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; HeLa Cells; High Mobility Group Proteins/biosynthesis; High Mobility Group Proteins/genetics; Histones/genetics; Histones/metabolism; Humans; Nucleosomes/genetics; Oxidative Stress/genetics; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Transcription Factors/genetics; Transcription, Genetic; Transcriptional Elongation Factors/biosynthesis; Transcriptional Elongation Factors/genetics; Uracil/metabolism; Xenopus laevis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:SSRP1		GO:1902275: regulation of chromatin organization	ECO:0000314:		P		In Figure 3, The presence of FACT increases the efficiency of RSC-induced nucleosome mobilization.	complete CACAO 12576

References

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