PMID:270715

Citation	Kinnunen, PK, Jackson, RL, Smith, LC, Gotto, AM Jr and Sparrow, JT (1977) Activation of lipoprotein lipase by native and synthetic fragments of human plasma apolipoprotein C-II. Proc. Natl. Acad. Sci. U.S.A. 74:4848-51
Abstract	Apolipoprotein C-II (apoC-II), a protein constituent of human very low density lipoproteins, is the activator for lipoprotein lipase (LPL; triacylglycerol acyl-hydrolase, EC 3.1.1.3). The amino acid sequence of the 78 residues of apoC-II has recently been established in this laboratory. To determine the minimal sequence requirements for activation, we have prepared both native and synthetic fragments of apoC-II and tested them for their ability to activate LPL. Cyanogen bromide fragments of apoC-II corresponding to residues 1--9 and 10--59 had little ability to activate LPL. However, the COOH-terminal cyanogen bromide fragment corresponding to residues 60--78 increased hydrolysis 4-fold compared to an average of 9-fold activation for the same concentration of apoC-II. The synthetic peptide containing residues 60--78 prepared by solid-phase techniques enhanced the lipolysis 3-fold. Addition of five residues produced a synthetic fragment 55--78 that enhanced the release of fatty acid 12-fold compared to 13-fold for intact apoC-II. By contrast, the synthetic peptide containing residues 66--78 did not activate. Removal of the three COOH-terminal residues, Gly-Glu-Glu, from fragment 60--78 decreased the ability to activate LPL by greater than 95%. These studies suggest that the maximal activation of LPL by apoC-II requires a minimal sequence contained within residues 55--78.
Links	PubMed PMC432053
Keywords	Amino Acid Sequence; Apolipoproteins/pharmacology; Chemical Phenomena; Chemistry; Cyanogen Bromide; Enzyme Activation/drug effects; Humans; Hydrofluoric Acid; Hydrolysis; Lipoprotein Lipase/metabolism; Peptide Fragments/chemical synthesis; Peptide Fragments/isolation & purification; Peptide Fragments/pharmacology; Structure-Activity Relationship
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:APOC2	enables	GO:0060230: lipoprotein lipase activator activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:APOC2		GO:0060230: lipoprotein lipase activator activity	ECO:0000314:		F		Based on figure 4 and figure 5 activity of Lipoprotein Lipase (LPL) was enhanced by Apolipoprotein C-II (apoC-II)	complete CACAO 6450

References

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