PMID:27058626

Citation	'Amin, FU, Shah, SA and Kim, MO (2016) Glycine inhibits ethanol-induced oxidative stress, neuroinflammation and apoptotic neurodegeneration in postnatal rat brain. Neurochem. Int. '
Abstract	Here we investigated for the first time the inhibitory potential of Glycine (Gly) against ethanol-induced oxidative stress, neuroinflammation and apoptotic neurodegeneration in human neuroblastoma SH-SY5Y cells and in the developing rat brain. The Gly co-treatment significantly increased the cell viability, inhibited the expression of phospho-Nuclear Factor kappa B (p-NF-kB) and caspase-3 and reduced the oxidative stress in ethanol-treated SH-SY5Y cells in a PI3K- dependent manner. Seven days old male rat pups were injected with ethanol (5 g/kg subcutaneously, prepared in a 20% saline solution) and Gly (1 g/kg). Gly co-treatment stimulated the PI3K/Akt signaling pathway to limit the ethanol induced reactive oxygen species (ROS) production in the developing rat brain. It lowered the ethanol-elevated levels of phospho-c Jun N terminal kinase (p-JNK) and its various downstream apoptotic markers, including Bax, cytochrome C, caspase-3 and PARP-1. Additionally, the Gly treatment upregulated antiapoptotic Bcl-2 proteins and prevented ethanol-induced neurodegeneration as assessed by Fluoro-Jade-B (FJB) and Nissl staining. Furthermore, the Gly administration caused significant reduction in the ethanol-induced neuroinflammation by inhibiting the expression of inflammatory markers such as p-NF-kB, cyclooxygenase 2 (COX2) and tumor necrosis factor-α (TNF-α) and reversed the ethanol-induced synaptic protein markers expression. The results suggest that acute Gly treatment reduces ethanol-induced oxidative stress and neuronal cell loss in SH-SY5Y cells and in the developing rat brain. Therefore, Gly may be considered as potential treatment in ethanol-intoxicated newborns and infants.
Links	PubMed Online version:10.1016/j.neuint.2016.04.001
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:GLRA1		GO:0060080: response to alcohol	ECO:0000250:	PMID:27058626 UniProtKB:P07727	P		The graphical display figure in the paper describes and explains the effect of glycine in rat's brains. The figure is also indirectly an info-graphic that helps in understanding the protein's function. Glycine receptors are ligand gated chloride channels that are found in a rat's brain. In this investigation, the researchers investigated the inhibitory potential of glycine against alcohol-induced oxidative stress, neuroinflammation and neurodegeneration in the rat brain. The researchers performed Gly co-treatment on the rat pups to investigate it's effects by injecting ethanol into the rat brains. They found that glycine reduced the alcohol induced stress and neuronal loss in the rat brain. Therefore with this finding, they concluded that glycine could be a potential treatment against alcohol-intoxication for newborns and infants.	complete CACAO 11575

References

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