PMID:2656641

Citation	Wachi, M and Matsuhashi, M (1989) Negative control of cell division by mreB, a gene that functions in determining the rod shape of Escherichia coli cells. J. Bacteriol. 171:3123-7
Abstract	Exponentially growing Escherichia coli cells containing additional copies of the shape-determining gene mreB were found to be elongated, whereas mreB mutant cells were spherical and overproduced penicillin-binding protein 3, a septum peptidoglycan synthetase. The effect of the mreB gene on expression of ftsI, the structural gene for penicillin-binding protein 3, was examined by using an ftsI-lacZ fusion gene on a plasmid. Formation of beta-galactosidase from the fusion gene was significantly increased in mreB129 mutant cells, and its overproduction was suppressed to a normal level by the presence of a plasmid containing the mreB gene. These results indicate a negative mechanism of control of cell division by this morphology gene and suggest that the gene functions in determining whether division or elongation of the cells occurs.
Links	PubMed PMC210024
Keywords	Cell Division; Cloning, Molecular; Escherichia coli/cytology; Escherichia coli/genetics; Gene Expression Regulation; Genes; Genes, Bacterial; Genes, Regulator
edit table

Significance

Gene product

Qualifier

GO Term

Evidence Code

with/from

Aspect

Extension

Notes

Status

involved_in

GO:0051782: negative regulation of cell division

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

GO:0051782: negative regulation of cell division

ECO:0000315:

P

Figure 4A. shows filamentous cell growth in strains carrying additional copies of mreB gene, a result of inhibition of cell division.

Table 1. shows overproduction of cell division gene ftsI in mreB mutant cells.

See Help:References for how to manage references in GONUTS.