GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:26364722
| Citation |
Singh, V, Azad, GK, Sariki, SK and Tomar, RS (2015) Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p. FEBS Lett. 589:3165-74 |
|---|---|
| Abstract |
The Nrd1-Nab3-Sen1 (NNS) complex terminates transcription of non-coding RNA genes and mediates degradation of the produced transcript by the nuclear exosome. The NNS complex also represses some stress response genes, by stimulating premature termination. A well-characterized stress response in yeast is flocculation, where cells aggregate to form flocs under expression of lectin-encoding genes designated as FLOs. In this study, we demonstrated the role of the NNS complex and Rrp6p in the expression of flocculation genes: FLO1, FLO5, FLO9, and FLO10. Furthermore, a deletion mutant of the RNA processing machinery (RNT1), and SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype. In summary, we have identified a cooperative role of Rnt1p, Rrp6p and the NNS complex in the repression of FLO genes. |
| Links |
PubMed Online version:10.1016/j.febslet.2015.09.006 |
| Keywords |
DNA Helicases/genetics; DNA Helicases/metabolism; Exosome Multienzyme Ribonuclease Complex/genetics; Exosome Multienzyme Ribonuclease Complex/metabolism; Flocculation; Gene Expression Regulation, Fungal; Lectins/genetics; Lectins/metabolism; Mannose-Binding Lectins/genetics; Mannose-Binding Lectins/metabolism; Models, Genetic; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phenotype; Protein Binding; RNA Helicases/genetics; RNA Helicases/metabolism; RNA, Fungal/genetics; RNA, Fungal/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III/genetics; Ribonuclease III/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0060257: negative regulation of flocculation |
ECO:0000315: |
P |
Flocculation was monitored through growth assays and was measured by rate of sedimentation. It was found that the amount of flocculation as well as the rate of flocculation was greater in the SEN1 mutants that in wild type cells (Figure 1A and 1B). |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
