GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:26299946
| Citation |
Sung, TC, Li, CY, Lai, YC, Hung, CL, Shih, O, Yeh, YQ, Jeng, US and Chiang, YW (2015) Solution Structure of Apoptotic BAX Oligomer: Oligomerization Likely Precedes Membrane Insertion. Structure 23:1878-1888 |
|---|---|
| Abstract |
Proapoptotic BAX protein is largely cytosolic in healthy cells, but it oligomerizes and translocates to mitochondria upon receiving apoptotic stimuli. A long-standing challenge has been the inability to capture any structural information beyond the onset of activation. Here, we present solution structures of an activated BAX oligomer by means of spectroscopic and scattering methods, providing details about the monomer-monomer interfaces in the oligomer and how the oligomer is assembled from homodimers. We show that this soluble oligomer undergoes a direct conversion into membrane-inserted oligomer, which has the ability of inducing apoptosis and structurally resembles a membrane-embedded oligomer formed from BAX monomers in lipid environment. Structural differences between the soluble and the membrane-inserted oligomers are manifested in the C-terminal helices. Our data suggest an alternative pathway of apoptosis in which BAX oligomer formation occurs prior to membrane insertion. |
| Links |
PubMed Online version:10.1016/j.str.2015.07.013 |
| Keywords |
Amino Acid Sequence; Apoptosis/genetics; Binding Sites; Cell Line; Cell Membrane/chemistry; Cell Membrane/metabolism; Crystallography, X-Ray; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Humans; Mitochondria/chemistry; Mitochondria/metabolism; Models, Molecular; Molecular Sequence Data; Mutation; Protein Binding; Protein Multimerization; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; bcl-2-Associated X Protein/chemistry; bcl-2-Associated X Protein/genetics; bcl-2-Associated X Protein/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0042802: identical protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q07813-1 |
F |
Seeded From UniProt |
complete | ||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
