PMID:26261212

Citation	Paquet, N, Adams, MN, Leong, V, Ashton, NW, Touma, C, Gamsjaeger, R, Cubeddu, L, Beard, S, Burgess, JT, Bolderson, E, O'Byrne, KJ and Richard, DJ (2015) hSSB1 (NABP2/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway. Nucleic Acids Res. 43:8817-29
Abstract	The maintenance of genome stability is essential to prevent loss of genetic information and the development of diseases such as cancer. One of the most common forms of damage to the genetic code is the oxidation of DNA by reactive oxygen species (ROS), of which 8-oxo-7,8-dihydro-guanine (8-oxoG) is the most frequent modification. Previous studies have established that human single-stranded DNA-binding protein 1 (hSSB1) is essential for the repair of double-stranded DNA breaks by the process of homologous recombination. Here we show that hSSB1 is also required following oxidative damage. Cells lacking hSSB1 are sensitive to oxidizing agents, have deficient ATM and p53 activation and cannot effectively repair 8-oxoGs. Furthermore, we demonstrate that hSSB1 forms a complex with the human oxo-guanine glycosylase 1 (hOGG1) and is important for hOGG1 localization to the damaged chromatin. In vitro, hSSB1 binds directly to DNA containing 8-oxoguanines and enhances hOGG1 activity. These results underpin the crucial role hSSB1 plays as a guardian of the genome.
Links	PubMed PMC4605301 Online version:10.1093/nar/gkv790
Keywords	Ataxia Telangiectasia Mutated Proteins/metabolism; Cell Survival; Chromatin/enzymology; Chromatin/metabolism; DNA Adducts/metabolism; DNA Glycosylases/metabolism; DNA Repair; DNA-Binding Proteins/metabolism; DNA-Binding Proteins/physiology; Guanine/analogs & derivatives; Guanine/metabolism; HeLa Cells; Humans; Mitochondrial Proteins/metabolism; Mitochondrial Proteins/physiology; Oxidative Stress
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:SOSB1		GO:0071168: protein localization to chromatin	ECO:0000314:		P		In figure 1, hSSB1 localizes to chromatin following oxidative stress.	complete CACAO 12559

References

See Help:References for how to manage references in GONUTS.