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PMID:2578891
| Citation |
Thacher, SM and Rice, RH (1985) Keratinocyte-specific transglutaminase of cultured human epidermal cells: relation to cross-linked envelope formation and terminal differentiation. Cell 40:685-95 |
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| Abstract |
The predominant form of the cross-linking enzyme, transglutaminase, in cultured normal human epidermal keratinocytes, is found in cell particulate material and can be solubilized by nonionic detergent. It elutes as a single peak upon either anion-exchange or gel-filtration chromatography. Monoclonal antibodies raised to the particulate enzyme cross-react with one of two transglutaminases in the cell cytosol. The second cytosolic transglutaminase, which has distinct kinetic and physical properties from the first, does not cross-react and is not essential for formation of the keratinocyte cross-linked envelope in vitro. The anti-transglutaminase antibodies stain the more differentiated layers of epidermis in a pattern similar to that given by anti-involucrin antiserum. These observations support the hypothesis that the transglutaminase so identified is involved in cross-linked envelope formation in vivo. |
| Links | |
| Keywords |
Acyltransferases/immunology; Acyltransferases/isolation & purification; Acyltransferases/metabolism; Animals; Antibodies, Monoclonal/immunology; Cell Differentiation; Cell Line; Cell Membrane/enzymology; Cell Membrane/ultrastructure; Chromatography; Cytosol/enzymology; Epidermis/enzymology; Epidermis/ultrastructure; Epitopes/immunology; Female; Histocytochemistry; Humans; Hybridomas/immunology; Keratins/metabolism; Kinetics; Mice; Mice, Inbred BALB C; Transglutaminases |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031226: intrinsic to plasma membrane |
ECO:0000314: |
C |
Figure 2, 3, 4: distribution of TGM activity |
complete | ||||
|
part_of |
GO:0031226: intrinsic component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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