PMID:25653215

Citation	'Banglmaier, RF, Sander, EA and VandeVord, PJ (2015) Induction and quantification of collagen fiber alignment in a three-dimensional hydroxyapatite-collagen composite scaffold. Acta Biomater '
Abstract	Hydroxyapatite-collagen composite scaffolds are designed to serve as a regenerative load bearing replacement that mimics bone. However, the material properties of these scaffolds are at least an order of magnitude less than that of bone and subject to fail under physiological loading conditions. These scaffolds compositionally resemble bone but they do not possess important structural attributes such as an ordered arrangement of collagen fibers, which is a correlate to the mechanical properties in bone. Furthermore, it is unclear how much ordering of structure is satisfactory to mimic bone. Therefore, quantitative methods are needed to characterize collagen fiber alignment in these scaffolds for better correlation between the scaffold structure and the mechanical properties. A combination of extrusion and compaction was used to induce collagen fiber alignment in composite scaffolds. Collagen fiber alignment, due to extrusion and compaction, was quantified from polarized light microscopy images with a Fourier transform image processing algorithm. The Fourier transform method was capable of resolving the degree of collagen alignment from polarized light images. Anisotropy indices of the image planes ranged from 0.08 to 0.45. Increases in the degree of fiber alignment induced solely by extrusion (0.08 to 0.25) or compaction (0.25 to 0.44) was not as great as that by the combination of extrusion and compaction (0.35 to 0.45). Additional measures of randomness and fiber direction corroborate these anisotropy findings. This increased degree of collagen fiber alignment was induced in a preferred direction that is consistent with the extrusion direction and parallel with the compacted plane.
Links	PubMed Online version:10.1016/j.actbio.2015.01.033
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:CO2A1	Contributes to	GO:0005202: collagen	ECO:0000314:		F		Load bearing replacement that mimics bone	complete CACAO 10559

References

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