GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:25335891
| Citation |
Chapard, C, Meraldi, P, Gleich, T, Bachmann, D, Hohl, D and Huber, M (2014) TRAIP is a regulator of the spindle assembly checkpoint. J. Cell. Sci. 127:5149-56 |
|---|---|
| Abstract |
Accurate chromosome segregation during mitosis is temporally and spatially coordinated by fidelity-monitoring checkpoint systems. Deficiencies in these checkpoint systems can lead to chromosome segregation errors and aneuploidy, and promote tumorigenesis. Here, we report that the TRAF-interacting protein (TRAIP), a ubiquitously expressed nucleolar E3 ubiquitin ligase important for cellular proliferation, is localized close to mitotic chromosomes. Its knockdown in HeLa cells by RNA interference (RNAi) decreased the time of early mitosis progression from nuclear envelope breakdown (NEB) to anaphase onset and increased the percentages of chromosome alignment defects in metaphase and lagging chromosomes in anaphase compared with those of control cells. The decrease in progression time was corrected by the expression of wild-type but not a ubiquitin-ligase-deficient form of TRAIP. TRAIP-depleted cells bypassed taxol-induced mitotic arrest and displayed significantly reduced kinetochore levels of MAD2 (also known as MAD2L1) but not of other spindle checkpoint proteins in the presence of nocodazole. These results imply that TRAIP regulates the spindle assembly checkpoint, MAD2 abundance at kinetochores and the accurate cellular distribution of chromosomes. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control. |
| Links |
PubMed Online version:10.1242/jcs.152579 |
| Keywords |
Anaphase; Chromosomes, Human/metabolism; Gene Knockdown Techniques; HeLa Cells; Humans; Kinetochores/metabolism; M Phase Cell Cycle Checkpoints; Mad2 Proteins/metabolism; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Colocalizes with |
GO:0000794: condensed nuclear chromosome |
ECO:0000314: |
C |
Immunofluorescence revealed that TRAIP localized at the periphery of chromosomes (Figure 1). |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
