PMID:2531656

Citation	Dal Toso, R, Sommer, B, Ewert, M, Herb, A, Pritchett, DB, Bach, A, Shivers, BD and Seeburg, PH (1989) The dopamine D2 receptor: two molecular forms generated by alternative splicing. EMBO J. 8:4025-34
Abstract	Cloned human dopamine D2 receptor cDNA was isolated from a pituitary cDNA library and found to encode an additional 29 amino acid residues in the predicted intracellular domain between transmembrane regions 5 and 6 relative to a previously described rat brain D2 receptor. Results from polymerase chain reactions as well as in situ hybridization revealed that mRNA encoding both receptor forms is present in pituitary and brain of both rat and man. The larger form was predominant in these tissues and, as shown in the rat, expressed by dopaminergic and dopaminoceptive neurons. Analysis of the human gene showed that the additional peptide sequence is encoded by a separate exon. Hence, the two receptor forms are generated by differential splicing possibly to permit coupling to different G proteins. Both receptors expressed in cultured mammalian cells bind [3H]spiperone with high affinity and inhibit adenylyl cyclase, as expected of the D2 receptor subtype.
Links	PubMed PMC401577
Keywords	Amino Acid Sequence; Autoradiography; Base Sequence; Cloning, Molecular; DNA/genetics; DNA/isolation & purification; Exons; Genes; Genetic Vectors; Humans; Molecular Sequence Data; Mutation; Nucleic Acid Hybridization; Oligonucleotide Probes; Pituitary Gland/metabolism; Plasmids; Polymerase Chain Reaction; RNA Splicing; RNA, Messenger/analysis; RNA, Messenger/genetics; Receptors, Dopamine/genetics; Receptors, Dopamine D2; Restriction Mapping; Sulfur Radioisotopes
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:DRD2	involved_in	GO:0007195: adenylate cyclase-inhibiting dopamine receptor signaling pathway	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:DRD2	enables	GO:0001591: dopamine neurotransmitter receptor activity, coupled via Gi/Go	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HUMAN:DRD2	part_of	GO:0005887: integral component of plasma membrane	ECO:0000305: curator inference used in manual assertion	GO:0001591	C		Seeded From UniProt	complete
HUMAN:DRD2	involved_in	GO:0007194: negative regulation of adenylate cyclase activity	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete

References

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