PMID:25008318

Citation	Noda, C, Kimura, H, Arasaki, K, Matsushita, M, Yamamoto, A, Wakana, Y, Inoue, H and Tagaya, M (2014) Valosin-containing protein-interacting membrane protein (VIMP) links the endoplasmic reticulum with microtubules in concert with cytoskeleton-linking membrane protein (CLIMP)-63. J. Biol. Chem. 289:24304-13
Abstract	The distribution and morphology of the endoplasmic reticulum (ER) in mammalian cells depend on both dynamic and static interactions of ER membrane proteins with microtubules (MTs). Cytoskeleton-linking membrane protein (CLIMP)-63 is exclusively localized in sheet-like ER membranes, typical structures of the rough ER, and plays a pivotal role in the static interaction with MTs. Our previous study showed that the 42-kDa ER-residing form of syntaxin 5 (Syn5L) regulates ER structure through the interactions with both CLIMP-63 and MTs. Here, we extend our previous study and show that the valosin-containing protein/p97-interacting membrane protein (VIMP)/SelS is also a member of the family of proteins that shape the ER by interacting with MTs. Depletion of VIMP causes the spreading of the ER to the cell periphery and affects an MT-dependent process on the ER. Although VIMP can interact with CLIMP-63 and Syn5L, it does not interact with MT-binding ER proteins (such as Reep1) that shape the tubular smooth ER, suggesting that different sets of MT-binding ER proteins are used to organize different ER subdomains.
Links	PubMed PMC4148859 Online version:10.1074/jbc.M114.571372
Keywords	Amino Acid Sequence; Cytoplasm/metabolism; Endoplasmic Reticulum/metabolism; HEK293 Cells; Humans; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Molecular Sequence Data; Selenoproteins/chemistry; Selenoproteins/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:SELS		GO:0005789: endoplasmic reticulum membrane	ECO:0000315:		C		Organism : homo sapiens Protein: VIMP // Selenos Figure 1B shows the localization of the protein to the ER membrane.	complete CACAO 12875

References

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