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PMID:24678929
| Citation |
Ghose, K, Selvaraj, K, McCallum, J, Kirby, CW, Sweeney-Nixon, M, Cloutier, SJ, Deyholos, M, Datla, R and Fofana, B (2014) Identification and functional characterization of a flax UDP-glycosyltransferase glucosylating secoisolariciresinol (SECO) into secoisolariciresinol monoglucoside (SMG) and diglucoside (SDG). BMC Plant Biol. 14:82 |
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| Abstract |
Lignans are a class of diphenolic nonsteroidal phytoestrogens often found glycosylated in planta. Flax seeds are a rich source of secoisolariciresinol diglucoside (SDG) lignans. Glycosylation is a process by which a glycosyl group is covalently attached to an aglycone substrate and is catalyzed by uridine diphosphate glycosyltransferases (UGTs). Until now, very little information was available on UGT genes that may play a role in flax SDG biosynthesis. Here we report on the identification, structural and functional characterization of 5 putative UGTs potentially involved in secoisolariciresinol (SECO) glucosylation in flax. |
| Links |
PubMed PMC3986616 Online version:10.1186/1471-2229-14-82 |
| Keywords |
Amino Acid Motifs; Amino Acid Sequence; Biosynthetic Pathways/genetics; Butylene Glycols/chemistry; Butylene Glycols/metabolism; Chromatography, High Pressure Liquid; Cloning, Molecular; Conserved Sequence; DNA, Complementary/genetics; Enzyme Assays; Expressed Sequence Tags; Flax/enzymology; Flax/genetics; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Glucosides/chemistry; Glucosides/genetics; Glucosides/metabolism; Glycosylation; Glycosyltransferases/chemistry; Glycosyltransferases/genetics; Glycosyltransferases/metabolism; Kinetics; Lignans/chemistry; Lignans/metabolism; Mass Spectrometry; Molecular Sequence Data; Organ Specificity/genetics; Oxidoreductases/genetics; Oxidoreductases/metabolism; Phylogeny; Sesamum/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016757: transferase activity, transferring glycosyl groups |
ECO:0000314: |
F |
Figure 8 shows the activity of UGT74S1 on SECO as a substrate. Panel A is the negative control with no enzyme present. The only peak corresponds to SECO. Panel B shows the peaks formed when 50 microliters of crude UGT74S1 is added. The additional peaks correspond to the standards for SMG and SDG, as shown in panels D and E., implying transfer of the glucose from UDP-glucose to SECO. Figure 9 confirms this with purified enzyme. |
complete | ||||
Notes
See also
References
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